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Category: Volume 07 Issue 04 April 2019
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Title: Comparative assessment of Cartridge based Nucleic Acid amplification Technique with Microscopy among Pulmonary TB Patients in a Tertiary care Hospital of Tripura

Authors: Dr Pradip Kumar Das, Dr. Niladri Sekhar Das, Dr Jayanta Debnath, Dr Anup Saha

 DOI: https://dx.doi.org/10.18535/jmscr/v7i4.171

Abstract

In the era of rapid diagnosis of TB and Drug resistance Cartridge Based Nucleic Acid Amplification Technique (CBNAAT) came with a future of early and easy diagnosis for TB. However Microscopic examination of sputum sample still is the most commonly used screening test and Lowenstein Jensen (LJ) culture method considered as a gold standard technique. Hence a study was planned to see the Concordance and efficacy of CBNAAT with smear Microscopy at TMC and Dr BRAM teaching Hospital Hapania Tripura from January to December 2017.

Material and Methods: CBNNAT and smear microscopy of 278 TB suspected patients were done ion the department of Microbiology.

Results: Smear positive cases CBNAAT positivity was 95.91% and smear negative cases 10.48%. Concordance of two techniques (CBNAAT and Microscopy) for the diagnosis of Tuberculosis was noticed to be 90.64% . 74.55% of cases were found to be negative by CBNAAT. Total 72 cases were positive for MTb and 4 cases were Rifampicin resistance.

Conclusion: CBNAAT positive result suggest but negative result do not exclude TB. CBNAAT does not eliminate the need of conventional microscopy however LJ should be establish soon that are required to monitor the CBNAAT negative cases and  to detect resistance to drugs other than Rifampicin.

Keywords: CBNAAT , Sputum Microscopy, Drug  resistance. 

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Corresponding Author

Dr Niladri Sekhar Das

TMC and DR BRAM Teaching Hospital, Hapania Agartala, Tripura, India

Phone No 8974816077, Email This email address is being protected from spambots. You need JavaScript enabled to view it.