Title: Speciation, Biofilm production and Antibiotic resistance pattern of Coagulase negative Staphylococci isolated from Neonatal septicemia
Authors: Dr B. Senthilselvan, DCH, MDVL, Dr B. Subitha MD
DOI: https://dx.doi.org/10.18535/jmscr/v7i1.116
Abstract
Nowadays Coagulase-Negative Staphylococci (CoNS) has become one of the major pathogen for neonatal septicemia as a result of increased use of intravascular devices and other invasive procedures. This prospective study was conducted to study about species distribution, biofilm production and antibiotic resistance pattern of Coagulase-Negative Staphylococci isolated from Neonatal Septicemia. During the ten months study period 68 CoNS were isolated from neonatal septicemia. Speciation of CONS was done by simple, inexpensive biochemical tests and biofilm production was detected by Tube Method and Congo red agar method. On speciation of CoNS Staphylococcus epidermidis (53%) was the common isolate followed by Staphylococcus haemolyticus (41%), Staphylococcus warneri (3%) and Staphylococcus schleiferi (3%) CoNS isolates showed significant resistance to Ampicillin (82%),Clindamycin(76%), Cotrimoxazole (74%) Erythromycin (74%), and Methicillin (76%.) .CoNS isolates revealed 100% sensitivity to Vancomycin. CoNS isolates (53%) were biofilm producers by Congo Red Agar method and (47%) were biofilm producers by Tube adherence method. Due to their high antibiotic resistance and increased virulence of CoNS leads to increased morbidity and mortality in neonates. Preventive measures like hand hygiene, aseptic management of intravenous line should be followed to reduce the spread of virulent CoNS.
Keywords: CoNS, Speciation, Biofilm production.
References
- Shivanna, Sunkappa, The rising trend of coagulase-negative staphylococci in neonatal septicemia, Indian Journal of Pathology and Microbiology, Volume59/ issue 4 pages 510-514
- J L Yu, S X Wu, H Q Jia, Study on Antimicrobial Susceptibility of Bacteria Causing Neonatal Infections: A 12 Year Study (1987 - 1998) Singapore Med J 2001 Vol 42(3) : 107-110
- Shreya Singh, Sujeesh Sebastian, Benu Dhawan, The Changing Face of Coagulase-Negative Staphylococci:Diagnostic And Therapeutic Challenges, Manipal Journal of Medical Sciences | June 2016 | Volume 1, 29-37.
- Klingenberg C1, Aarag E, Rønnestad A, Sollid JE, Abrahamsen, Coagulase-negative staphylococcal sepsis in neonates. Association between antibiotic resistance, biofilm formation and the host inflammatory response. Pediatr Infect Dis J. 2005 Sep;24(9):817-22.
- SarojGolia, Deepali Bhimacharya Telsang, Asha S. Kamath B, Speciation of clinically significant coagulase negative staphylococci and their antibiotic resistant patterns in a tertiary care hospital, International Journal of Research in Medical Sciences, 2015 May;3(5):1242-1246.
- Y. Asangi, Mariraj. J, Sathyanarayan. M.S, Speciation of clinically significant Coagulase Negative Staphylococci and their antibiotic resistant patterns in a tertiary care hospital. Int J Biol Med Res. 2011; 2(3): 735-739.
- Mila V.S, Dr.S.Manjusree, Sri. Ashraf Perilacode, Speciation of the Coagulase Negative Staphylococci Isolated from Various Clinical Samples and their Antimicrobial Resistance Pattern at a Tertiary Care Hospital, JMSCR Vol||05||Issue||03||Page 19063-19068|| March 2017.
- T Mathur, S Singhal, S Khan, DJ Upadhyay, T Fatma, A Rattan, Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods ,Indian Journal of Medical Microbiology, (2006) 24 (1):25-9,
- Riyaz Sheriff1, A Sheena2, Assessment of Biofilm Production in Clinically Significant Isolates of Staphylococcus epidermidis and Comparison of Qualitative and Quantitative Methods of Biofilm Production in a Tertiary Care Hospital, International Journal of Scientific Study |September 2016 | Vol 4 | Issue 6 41-46
- Bizzarro, Shabanova, Neonatal Sepsis 2004–2013: The Rise and Fall of Coagulase-Negative Staphylococci, The Journal of pediatric ,Volume 166,Issue 5, 2015 pages 1193-1199
- Elizabeth A. Marchant, Guilaine K. Boyce, Manish Sadarangani, and Pascal M. Lavoie,Neonatal Sepsis due to Coagulase-Negative Staphylococci Clinical and Developmental Immunology Volume 2013, 10 pages 586076
- S. Vijayasri Badampudi, Surya Kirani KRL, Rajyalakshmi, Coagulase Negative Staphylococcus; the Speciation and Biofilm Production of Coagulase Negative Staphylococcal Isolates from Clinically Significant Specimens and their Antibiogram. JKIMSU, Vol. 5, No. 2, April-June 2016 69-78.
- Usha MG1, Shwetha DC, Vishwanath G. Speciation of coagulase negative Staphylococcal isolates from clinically significant specimens and their antibiogram. Indian J Pathol Microbiol. 2013 Jul-Sep;56(3):258-60. doi: 10.4103/0377-4929.120383.
- Lok Bahadur Shrestha, Narayan Raj Bhattarai and BasudhaKhanal, Antibiotic resistance and biofilm formation among coagulase-negative staphylococci isolated from clinical samples at a tertiary care hospital of eastern Nepal. Antimicrobial Resistance & Infection Control2017 86:89
- Oliveira, De Lourdes Rs Cunha, Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci, Research Gate,2010,Volume/page260
- Martini, Hörner, Investigation of biofilm formation in coagulase-negative staphylococci isolated from platelet concentrate bags, Rev Inst Med Trop Sao Paulo,2016, Volume 58
- Shahsanam Gheibi, Mohammad Karamyyar, Most Common Cause of Neonatal Septicemia in Urmia, Iran, Iran J Pediatr Sep 2008; Vol 18 ( No 3), Pp:237-243.
- Astha Agarwal, Amita Jain, Association between drug resistance and production biofilim in Staphylococci, indian J Pathol Microbiol 2013 Jul-Sep:56(3)258-260.