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Category: Volume 07 Issue 01 January 2019
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Title: Absolute CD34+ Cell Count by Flow Cytometry at M.Y. Hospital Indore

Authors: Prof. Dr C.V. Kulkarni, Prof.  Dr A. Panchonia, Dr Sachin Sharma

 DOI: https://dx.doi.org/10.18535/jmscr/v7i1.101

Abstract

 

Introduction: An increasing number of medical centers can collect bone marrow, peripheral blood, stem cells. Pathology laboratories should accommodate this trend, but investment in additional equipment may be impractical. In concert with the International Society of Hematotherapy and Graft Engineering (ISHAGE), we previously described a set of guidelines for detection of CD34cells With this procedure, an absolute CD34 count is generated by incorporating the leukocyte count from an automated hematology analyser (two-platform method). In the present study, we modified the basic ISHAGE method with the addition of a known number of Flow-County fluorospheres. To reduce errors inherent to sample washing/centrifugation,

Aims: To compare CD34+ cell counting results by using with and without the use of a separate hematology analyzer (ie, single-platform versus dual-platform methodologies).

Material and Method: A total of 34 samples of peripheral blood, apheresis packs, and bone marrow aspiration were analysed and compared using the ISHAGE protocol with or without the addition of fluorescent microspheres. Dual-platform CD34+ cell counting incorporated data from navious modal 2 laser and eight colour.

Results: Subtle differences in CD34+ cell counting between 2 systems and 2 methods did not achieve statistical significance.

Conclusion: It is the accurate determination CD34+ cell enumeration, properly validated, can support care for patients undergoing transplants setting. These modifications may improve the inter laboratory reproducibility

Keywords: International Society of Hematotherapy and Graft Engineering (ISHAGE), Absolute CD34+ Cell COUNT.

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Corresponding Author

Dr Sachin Sharma

Assistant Professor MGM Medical College with M.Y. Hospital Indore, MP, India

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