Title: Glucose Estimation: The most suitable blood collection tube for glucose estimation

Authors: Hamdan Al-Hinai, Osama Al-Ghatreefy, Salim Al Jafari, Salim Al Hadabi, Dr Hassan Sadek

 DOI: https://dx.doi.org/10.18535/jmscr/v7i1.69

Abstract

 

Introduction: Patient with high blood sugar depend on accurate diagnosis glucose estimation for correct diagnosis, therefore antiglycolysis are used in collection tube to stabilize the glucose level in the tube.

Methods: collecting articles from different databases that related to stability of glucose concentration in blood collection tubes. This study aimed to find out what is the best tube for glucose estimation.

Conclusion: The mixture tubes (venosafe, gluco EXACT and Glucomedic) show the best results for glucose stability and the second tube is the citrate tube.

Keywords: glucose estimation, antiglcolysis, anticoagulant, blood collection tube, NaF/KOx, citrate, Glucomedics.

References

  1. American Diabetes Association. (2018). Statistics About Diabetes. [online] Available at: http://www.diabetes.org/diabetes-basics/statistics/ [Accessed 30 Apr. 2018].
  2. Bueding, E, Goldfarb, W. The effect of sodium fluoride and sodium iodoacetate on glycolysis in human blood. J Biol Chem 1941; 14: 539–44
  3. B. Sacks, M. Arnold, G.L. Bakris, D.E. Bruns, A.R. Horvath, M.S. Kirkman, et al., Executive summary: guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus, Clin. Chem. 57 (2011) 793–798.
  4. co.uk. (2018). What is HbA1c? - Definition, Units, Conversion, Testing & Control. [online] Available at: https://www.diabetes.co.uk/what-is-hba1c.html [Accessed 13 May 2018].
  5. Dimeski, G., Yow, K. and Brown, N. (2015). What is the most suitable blood collection tube for glucose estimation?. Annals of Clinical Biochemistry, 52(2), pp.270-275.
  6. A. Amegashie1, N. Amidu2 and W.K.B.A. Owiredu3 (2015). A comparison of fluoride-oxalate and plain (serum gel) tube on glu-cose measurement. Medical and Biomedical Sciences, 4(1), pp.34-40.
  7. Fernandez L, Jee P, Klein MJ, et al. A comparison of glucose concentration in paired specimens collected in serum separator and fluoride/potassium oxalate blood collection tubes under survey ‘field’ conditions. Clin Biochem 2013; 46: 285–288.
  8. Fobker, M. (2014). Stability of glucose in plasma with different anticoagulants. Clinical Chemistry and Laboratory Medicine (CCLM), 52(7).
  9. Lippi, G. Targher, Haemoglobin A1c and diagnosis of diabetes, not ready for the prime time? Ann. Clin. Biochem. 49 (2012) 508.
  10. Garcia del Pino I, Constanso I, Mourı´n LV, et al. Citric/ citrate buffer: an effective antiglycolytic agent. Clin Chem Lab Med 2013; 51: 1943–1949.
  11. G. del Pino, I. Constanso, L.V. Mourı´n, C.B. Safont, P.R. Va´zquez, Citric/citrate buffer: an effective antiglycolytic agent, Clin. Chem. Lab. Med. 51 (2013) 1943–1949.
  12. M. Mikesh, D.E. Bruns, Stabilization of glucose in blood specimens: mechanism of delay in fluoride inhibition of glycolysis, Clin. Chem. 54 (2008) 930–932.
  13. Lawrence J.M., Contreras R, Chen W.and Sacks D.A. (2008).Trends in the prevalence of pre -existing diabetes and gestational diabetes mellitus among a racially/ethnically diverse population of pregnant women. Diabetes Care 31(5):899-904.
  14. Li G, Cabanero M, Wang Z, et al. Comparison of glucose determination on blood samples collected in three types of tubes. Ann Clin Lab Sci 2013; 43: 278–284.
  15. Koseoglu, A. Hur, A. Atay, S. Cuhadar, Effects of hemolysis interference on routine biochemistry parameters, Biochem. Med. (Zagreb) 21 (2011) 79–85.
  16. Montagnana, G. Lippi, Overcoming preanalytical issues for diagnosing diabetes with fasting plasma glucose, Ann. Transl. Med. 5 (2017) 257.
  17. J. Peake, D.E. Bruns, D.B. Sacks, A.R. Horvath, It’s time for a better blood collection tube to improve the reliability of glucose results, Diabetes Care 36 (2013) e2.
  18. Ministry of Health Oman Annual Health Report 2008. From: http://www.moh.gov.om/stat/2008/index.htm. Accessed: 7 May 2018.
  19. News Today. (2018). Diabetes: Symptoms, causes, and treatments. [online] Available at: https://www.medicalnewstoday.com/info/diabetes [Accessed 28 Apr. 2018].
  20. Ridefelt, P., Åkerfeldt, T. and Helmersson-Karlqvist, J. (2014). Increased plasma glucose levels after change of recommendation from NaF to citrate blood collection tubes. Clinical Biochemistry, 47(7-8), pp.625-628.
  21. Roglic, G. (2016). WHO Global report on diabetes: A summary. International Journal of Non communicable Diseases, 1(1), p.3.
  22. Pasqualetti, D. Szo˝ke, S. Birindelli, A. Dolci, M. Panteghini, Optimal collection tubes for plasma glucose determination: confusion reigns supreme, Clin. Chem. Lab Med. 54 (2016) e281–283.
  23. Sacks DB, Arnold M, Bakris GL, et al. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Clin Chem 2011; 57: 793–798.

Corresponding Author

Dr Hassan Sadek

Medical Laboratory Sciences, Oman College of Health Sciences