Title: Epidemiology of Staphylococcus aureus in a burn unit of a tertiary care center in Bhubaneswar

Authors: Susant Mishra, Jayant Kumar Das

 DOI: https://dx.doi.org/10.18535/jmscr/v6i12.187

Abstract

Background: In creating nations, hospitalized consume exploited people are at high danger of nosocomial contaminations caused by Staphylococcus aureus. Hazard factors incorporate poor contamination control rehearses, delayed hospitalization and restricted limit with regards to research center microbiological examinations. These issues are exacerbated by boundless utilization of anti-infection agents that drives the spread of multidrug safe microbes.

Methods: Amid the investigation time frame (November 2014-June 2015), nasal and intrusive S. aureus disengages were gathered sequentially from patients and human services specialists (HCWs) inside the consume unit of the Reconstructive Plastic Surgery and Burn Center of Korle Bu Teaching Hospital in Ghana. Anti-microbial remedy, anti-microbial weakness and bacterial composing were utilized to evaluate anti-microbial weight, anti-toxin obstruction, and conceivable transmission occasions among patients and HCWs.

Results: Eighty S. aureus confines were gotten from 37 of the 62 included consume patients and 13 of the 29 HCWs. At affirmation, half of patients conveyed or were tainted with S. aureus including ethicillin safe S. aureus (MRSA). Anti-toxin use per 100 days of hospitalization was high (91.2 days), demonstrating high particular weight for safe pathogens. MRSA segregates got from 11 patients and one HCW had a place with a similar spa-type t928 and multi-locus grouping type 250, inferring conceivable transmission occasions. A death rate of 24% was recorded over the season of affirmation in the consume unit.

Conclusion: This examination uncovered a high potential for MRSA episodes and development of safe pathogens among consume patients because of absence of patient screening and expanded exact utilization of anti-infection agents. Our perceptions underscore the need to actualize an arrangement of anti-toxin stewardship and disease counteractive action where microbiological diagnostics results are made accessible to doctors for convenient and suitable patient treatment.

Keywords: MRSA, VRSA, S. aureus, Antibiotic resistance, Burn

References

  1. Barber M. Methicillin-resistant staphylo-cocci. J Clin Pathol 1961; 14: 385–393.
  2. Barrett FF, McGehee RF Jr, Finland M. Methicillin-resistant Staphylococcus aureus at Boston City Hospital: bacter-iologic and epidemiologic observations. N Engl J Med 1968; 279:441–448.
  3. Haley RW, Hightower AW, Khabbaz RF, et al. The emergence of methicillin-resistant Staphylococcus aureus infections in United States hospitals. Possible role of the house staff-patient transfer circuit. Ann Intern Med 1982; 97:297–308.
  4. Jarvis WR, Schlosser J, Chinn RY, Tweeten S, Jackson M. National prevalence of methicillin-resistant Staphylococcus aureus in inpatients in US health care facilities, 2006. Am J Infect Control 2007; 35:631–637.
  5. Cosgrove SE, Sakoulas G, Perencevich EN, Schwaber MJ, Karchmer AW, Carmeli Y. Comparison of mortality associated with methicillin resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin Infect Dis 2003; 36:53–59.
  6. Klevens RM, Morrison MA, Nadle J, et al. Invasivemethicillin-resistant Staphylo-coccus aureus infections in the United Status. JAMA 2007; 298: 1763–1771.
  7. Bancroft EA. Antimicrobial resistance: it’s not just for hospitals. JAMA 2007; 298:1803–1804.
  8. Centers for Disease Control and Prevention. HIV/AIDS Surveillance Report, 2005. Vol. 17. Rev ed. Atlanta: US Department of Health and Human Services, Centers for Disease Control and Prevention; 2007: 16. http://www.cdc.gov/hiv/topics/surveillance/resources/reports/. Accessed 30 October 2009.
  9. Diep BA, Sensabaugh GF, Somboona NS, Carleton HA, Perdreau-Remington F. Widespread skin and soft-tissue infections due to two methicillin- resistant Staphylococcus aureus strains harboring the genes for Panton-Valentine leucocidin. J Clin Microbiol 2004; 42:2080–2084.
  10. Okuma K, Iwakawa K, Turnidge JD, et al. Dissemination of new methicillin- resistant Staphylococcus aureus clones in the community. J Clin Microbiol 2002; 40:4289–4394.
  11. Purcell K, Fergie JE. Exponential increase in community-acquired methicillin-resistant Staphylococcus aureus infections in South Texas children. Pediatr Infect Dis J 2002; 21:988–989.
  12. Kollef MH, Micek ST. Methicillin-resistant Staphylococcus aureus: a new community-acquired pathogen? Curr Opin Infect Dis 2006; 19: 161–168.
  13. Wang JL, Chen SY, Wang JT, et al. Comparison of both clinical features and mortality risk associated with bacteremia due to community-acquired methicillin-resistant Staphylococcus aureus and methicillin-susceptible aureus. Clin Infect Dis 2008; 46:799–806.
  14. Brakstad OG, Aasbakk K, Maeland JA (1992) Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc J Clin Microbiol 30: 1654±1660. PMID: 1629319
  15. Noto MJ, Kreiswirth BN, Monk AB, Archer GL (2008) Gene acquisition at the insertion site for SCCmec, the genomic island conferring methicillin resistance in Staphylococcus aureus. J Bacteriol 190: 1276± 1283. https://doi.org/10.1128/JB.01128-07 PMID: 18083809
  16. Clinical and laboratory Standard Institute (2010) Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement. CLSI document M100-S20. Wayne P., editor.
  17. Prevost G, Couppie P, Prevost P, Gayet S, Petiau P, Cribier B, et al. (1995) Epidemiological data on Staphylococcus aureus strains producing synergohymenotropic toxins. J Med Microbiol 42: 237±245. https://doi.org/10.1099/00222615-42-4-237 PMID: 7707330
  18. Harmsen D, Claus H, Witte W, RothgaÈnger J, Claus H, Turnwald D, et al. (2003) Typing of methicillin resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 41: 5442±5448. https://doi.org/10.1128/ JCM.41.12.5442-5448.2003 PMID: 14662923
  19. Enright MC, Day NPJ, Davies CE, Peacock SJ, Spratt BG (2000) Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38: 1008±1015. PMID: 10698988
  20. Jeschke M, Pinto R, Costford S, Amini-Nik S (2016) Threshold age and burn size associated with poor outcomes in the elderly after burn injury. Burns 42: 276±281. https://doi.org/10.1016/j.burns.2015.12. 008 PMID: 26803373
  21. Scholten-Jaegers SM, Nieuwenhuis M, van Baar M, Niemeijer A, Hiddingh J, Beerthuizen G, et al. (2017) Epidemiology and Outcome of Patients With Burns Treated With Cerium Nitrate Silversulfadiazine. J Burn Care Res 38: e432±e442. https://doi.org/10.1097/BCR.0000000000000448 PMID: 27685809
  22. Nthumba PM (2016) Burns in sub-Saharan Africa: A review. Burns 42: 258±266. Available: http:// linkinghub.elsevier.com/retrieve/pii/S0305417915001163. https://doi.org/10.1016/j.burns.2015.04.006 PMID: 25981292
  23. Agbenorku P, Edusei A, Ankomah J (2011) Epidemiological study of burns in Komfo Anokye Teaching Hospital, 2006±2009. Burns 37: 1259±1264. https://doi.org/10.1016/j.burns.2011.04.014 PMID: 21726953
  24. Kypraios T, O'Neill PD, Huang SS, Rifas-Shiman SL, Cooper BS (2010) Assessing the role of undetected colonization and isolation precautions in reducing methicillin-resistant Staphylococcus aureus transmission in intensive care units. BMC Infect Dis 10: 29. https://doi.org/10.1186/1471-2334-10-29 PMID: 20158891
  25. Merrer J, Santoli F, AppeÂre De Vecchi C, Tran B, De Jonghe B, Outin H (2000) ªColonization pressureº and risk of acquisition of methicillin-resistant Staphylococcus aureus in a medical intensive care unit. Infect Control Hosp Epidemiol 21: 718±723. Available: http://www.ncbi.nlm.nih.gov/pubmed/ 11089656. https://doi.org/10.1086/501721 PMID: 11089656
  26. Egyir B, Guardabassi L, Nielsen SS, Larsen J, Addo KK, Newman MJ, et al. (2013) Prevalence of nasal carriage and diversity of Staphylococcus aureus among inpatients and hospital staff at Korle Bu Teaching Hospital, Ghana. J Glob Antimicrob Resist 1: 189±193. https://doi.org/10.1016/j.jgar.2013.05.006 PMID: 27873611
  27. Enright MC, Robinson DA, Randle G, Feil EJ, Grundmann H, Spratt BG (2002) The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc Natl Acad Sci U S A 99: 7687±7692. Available http://www.ncbi.nlm.nih.gov/pubmed/12032344. https://doi.org/10.1073/pnas.122108599 PMID: 12032344
  28. Egyir B, Guardabassi L, Sørum M, Nielsen SS, Kolekang A, Frimpong E, et al. (2014) Molecular Epidemiology and Antimicrobial Susceptibility of Clinical Staphylococcus aureus from Healthcare Institutions in Ghana. PLoS One 9: e89716. Available: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid= 3934920&tool=pmcentrez&rendertype=abstract. https://doi.org/10.1371/journal.pone.0089716 PMID: 24586981
  29. Dekker D, Wolters M, Mertens E, Boahen K, Krumkamp R, Eibach D, et al. (2016) Antibiotic resistance and clonal diversity of invasive Staphylococcus aureus in the rural Ashanti Region, Ghana. BMC Infect Dis 16: 720. https://doi.org/10.1186/s12879-016-2048-3 PMID: 27899074
  30. Vandendriessche S, De Boeck H, Deplano A, Phoba M, Lunguya O, Falay D, et al. (2017) Characterisation of Staphylococcus aureus isolates from bloodstream infections, Democratic Republic of the Congo. Eur J Clin Microbiol Infect Dis https://doi.org/10.1007/s10096-017-2904-0 PMID: 28116552

Corresponding Author

Dr Jayant Kumar Das

Associate Professor, Dept of Plastic Surgery, IMS and SUM Hospital, Bhubaneswar, 751003, Odisha, India

Email: This email address is being protected from spambots. You need JavaScript enabled to view it.