Title: AMP C Producing Pseudomonas aeruginosa Isolated from a Tertiary Care Centre and its Association with Antibiotic Intake
Authors: Dr Meghana Vijayan P, Dr Joana Mary Magdaline
DOI: https://dx.doi.org/10.18535/jmscr/v6i11.151
Abstract
Pseudomonas aeruginosa is a commonly encountered gram negative bacteria which is a potential source of nosocomial infections. Amongst the various acquired mechanisms of resistance, production of beta lactamases is the most common. The failure to detect this may lead to therapeutic failure and spread of resistance .This study was conducted for a period of one year from March 2016 to find out the antibiotic susceptibility pattern of the Pseudomonas aeruginosa isolates obtained from various clinical samples received, to identify the proportion of Amp C producers among the isolates, and to find out whether there is an association between antibiotic intake and the emergence of antibiotic resistance. A total of 125 Pseudomonas aeruginosa isolates were obtained, majority were from respiratory samples. Most of the isolates (90%) were susceptible to Carbapenems. Among the 125 isolates, 110 screened positive as potential Amp C producers. Plasmid mediated Amp C production was confirmed by Boronic acid disk test whereas inducible Amp C was confirmed by disk antagonism test using Ceftazidime and the inducer, Imipenem. Out of the 110, 85 were confirmed to be Amp C producers and majority (81) were inducible Amp C producers and only 4 showed plasmid mediated Amp C production. A statistically significant association between antibiotic resistance and antibiotic usage was demonstrated. Hence steps to prevent inadvertent use of antibiotics should be taken to cut down the problem of resistance.
Keywords: Pseudomonas aeruginosa; plasmid mediated Amp C; inducible Amp C.
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