ABK is the metallic based herbal medicine, no scientific testimonial exist to conform its action and potential efficacy to proof the ancient siddhars’ quotation about Ayabirunkaraja katpam which is mentioned in reputed siddha text siddha vaithiya thirattu” whereas quoted it is best remedy for Anemia, grey hair and Weakness.

Powdered samples (10 g) were extracted with 100 ml of solvents (hexane, ethyl acetate, methanol, ethanol and water) and kept for 3 h at room temperature.  For antioxidant activity, the solvent was evaporated using a rotovapor (Make: Buchi, Model: R-300) and dry extract was obtained. The extract was then re-dissolved in water at 100 mg / ml ratio and used for the analysis. RBC membrane stabilization potential of plant extracts were investigated according to the methods proposed by Sakat et al. (2010). Human blood (2 ml) was drawn from volunteer in a heparinised tube and centrifuged at 2000 rpm for 10 min. The pellet (RBC cells) was washed twice with PBS (9 ml) and finally the pellet was re-suspended in 10 ml of PBS. The extract (500 µl) were added to 1 PBS, 1 ml of 3% H₂O₂ and incubated for 30 min. Based on the absorbance, the percentage of RBC membrane damage and inhibition of membrane damage were calculated.

It is concluded that the aqueous extract of Auabirungaraja karpam showed appreciable antioxidant and ferric reducing capacities and effective in preventing RBC membrane damage which due to oxidative stress.

Keywords: RBC, Antioxidant, Membrane.

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Varnakulendren N

PhD Research scholar, Department of Siddhamedicine, Tamil University, Thanjavur