Abstract
Drug replacement with appropriate remedies are required to eradicate existing problem in thrombosis. It rectified with partially purified thrombolytic enzyme from the seedling of Panicum sumatrance and Echinochola frumentacea. The cell free extract of P. Sumatrance and E. frumentacea seedling was partially purified by dialysis method and observed 36 and 38 KDa molecular weight protein. The isolated enzyme exhibited optimum blood clotting activity at pH 7 at 30⁰C. Thrombolytic model used to screen the clot lysis potential of bioactive metabolites and Proteolytic enzyme, extracted seedling. Streptokinase treatment was considered as confirmative and saline water as blank control for this study. The Extracts exhibited a good hemolysis activity and Anticoagulant effect with a maximum activity at 20µl concentration in in-vitro condition.
Keyword: P. sumatrance, E. frumentacea, Antithrombosis, Cardiac risk, Phytoremedy.
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Corresponding Author
A.J.A.Ranjith singh
Department of Biotechnology, Prathyusha Engineering College, Chennai- 602025, India
DOI: https://dx.doi.org/10.18535/jmscr/v6i10.100
