Background: Neonatal septicemia still a major cause of mortality and morbidity worldwide. Blood culture is the main stay in the diagnosis of septicemia. Emergence of drug resistant bacterial strains is a major problem in the management of sepsis.  Aim of present study is to determine bacteriological profile and antibiotic resistance in neonatal sepsis.

Method: The study was carried out in the Department of Microbiology, MGM, Medical College Indore during the period from January 2017 to June 2017.

450 blood samples collected from clinical suspected cases of bacteremia were studied. Growth from the subcultures were identified by standard biochemical testing and antibiotics resistance pattern were determined as per Clinical Laboratory Standard Institute (CLSI) guidelines.

Result: During that period, a total of 176 (39%) blood samples were found to be positive for bacterial isolates. Gram negative septicemia (50.5%) was encountered slightly more than gram positive septicemia (49.5%). The common bacteria isolated were Klebsiella spp., CONS, Staphylococcus aureus and Escherichia coli. Antibiotics resistance against gram negative bacilli were cefuroxime, ceftazidime, cefepime and ciprofloxacin. Gram-positive isolates showed excellent sensitivity to linezolid and vancomycin

Conclusion: The study concludes that the isolated organism exhibited higher resistance towards commonly used antimicrobials. Healthcare personnel and common population should also be aware of the rising antibiotic resistances to frequently used antibiotics.

Keywords: Antibiotic resistance, CONS, S. aureus, Neonatal Septicemia, Blood Culture

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Manish Purohit

Associate Professor, Department of Microbiology, M.G.M. Medical College, Indore, MP, India