Abstract
Background: Neonatal septicemia still a major cause of mortality and morbidity worldwide. Blood culture is the main stay in the diagnosis of septicemia. Emergence of drug resistant bacterial strains is a major problem in the management of sepsis. Aim of present study is to determine bacteriological profile and antibiotic resistance in neonatal sepsis.
Method: The study was carried out in the Department of Microbiology, MGM, Medical College Indore during the period from January 2017 to June 2017.
450 blood samples collected from clinical suspected cases of bacteremia were studied. Growth from the subcultures were identified by standard biochemical testing and antibiotics resistance pattern were determined as per Clinical Laboratory Standard Institute (CLSI) guidelines.
Result: During that period, a total of 176 (39%) blood samples were found to be positive for bacterial isolates. Gram negative septicemia (50.5%) was encountered slightly more than gram positive septicemia (49.5%). The common bacteria isolated were Klebsiella spp., CONS, Staphylococcus aureus and Escherichia coli. Antibiotics resistance against gram negative bacilli were cefuroxime, ceftazidime, cefepime and ciprofloxacin. Gram-positive isolates showed excellent sensitivity to linezolid and vancomycin
Conclusion: The study concludes that the isolated organism exhibited higher resistance towards commonly used antimicrobials. Healthcare personnel and common population should also be aware of the rising antibiotic resistances to frequently used antibiotics.
Keywords: Antibiotic resistance, CONS, S. aureus, Neonatal Septicemia, Blood Culture
References
- Lesser CF, Miller SI. Septicemia. In: Braunwald E, Hauser SL, Fauci AS, Longo DL, Kasper DL, Jameson JL, eds. Harrison's Principles of Internal Medicine. 15th McGraw-Hill Company, 2001; vol.1: 970-974.
- Tsai MH, Chu SM, Lee CW, Hsu JF, Huang HR, Chiang MC, et al. Recurrent late-onset sepsis in the neonatal intensive care unit: incidence, clinical characteristics and risk factors. Clin Microbiol Infect. 2014; 20(11): O928 – O935.
- Report of the National Neonatal Perinatal Database (National Neonatology Forum) 2002-03. New Delhi: National Neonato-logy Forum NNPD Network; 2005:70.
- Kumaravel K S, Rameshbabu B. A Study of the Bacteriological Profile and Antibiotic Sensitivity in Neonatal Septicemia. International Journal of Contemporary Medical Research. Volume 3 | Issue 6 | June 2016 | ICV: 50.43 |
- Meenakshi Kante*, Muni Lakshmi P, Sreenivasulu Reddy P Bacterial profile of blood stream infections and their antibiograms. Int J Res Med Sci. 2015 Mar;3(3):698-704.
- Kenneth C. Iregbu, Olufumilayo Y. Elegba, Iretiola B. Babaniyi Bacteriological profile of neonatal septicaemia in a tertiary hospital in Nigeria African Health Sciences 2006; 6(3): 151-154
- Kavitha Prabhu, Sevitha Bhat, Sunil Rao Bacteriologic Profile and Antibiogram of Blood Culture Isolates in a Pediatric Care Unit Journal of Laboratory Physicians / Jul-Dec 2010 / Vol-2 / Issue-2
- Murty D S, Gyaneshwari M. Blood cultures in paediatric patients: A study of clinical impact. Indian J Med Microbiol 2007;25:220-4
- Clinical and Laboratory Standards Institute [CLSI]. Performance for Antimicrobial disk Susceptibility Testing-Approved Standard. 27th information supplement. Wayne, PA. M100-S27; 2017.
- Mahapatra, A., Ghosh, S.K., Mishra, S., et al. 2002. Enterobacter cloacae: a predominant pathogen in neonatal septicaemia. Indian J. Med. Microbiol., 20: 110–12.
- Khanal B, Harish BN, Sethuraman KR, Srinivasan S. Infective endocarditis: report of prospective study in an Indian hospital. Trop Doct. 2002;32:83-5.
- Shrestha S, Shrestha NC, Singh S, Shrestha RPB, Kayestha S, Shrestha M et al. Bacterial isolates and its antibiotic susceptibility pattern in NICU. Kathmandu Univ Med J. 2013;41:66-70.
- Patel D, Nimbalkar A, Sethi A, Kungwani A, Nimbalkar S. Blood culture isolates in neonatal sepsis and their sensitivity in Anand district of India. Indian J Pediatr. 2014;81:785-90.
- Roy I, Jain A, Kumar M , Agarwal SK.Bacteriology of neonatal septicemia in a tertiary care hospital of northern India.IJMM 2002; 20:156-9.
- Bhat YR, Lewis LES Vandana KE. Bacterial isolates of early-onset neonatal sepsis and their antibiotic susceptibility pattern between 1998 and 2004: an audit from a center in India. Ital J Pediatr. 2011;37:32-7.
- Shrestha S, Shrestha NC, Singh S, Shrestha RPB, Kayestha S, Shrestha M et al. Bacterial isolates and its antibiotic susceptibility pattern in NICU. Kathmandu Univ Med J. 2013;41:66-70.
- Kumhar GD, Ramachandran VG, Gupta P. Bacteriological analysis of blood culture isolates from neonates in a tertiary care hospital in India. J Health Popul Nutr. 2002;20:343-7.
- van den Hoogen A, Gerards LJ, Verboon-Maciolek MA, Fleer A, Krediet TG. Long-term trends in the epidemiology of neonatal sepsis and antibiotic susceptibility of causative agents. Neonatology. 2010;97:22–8.
Corresponding Author
Manish Purohit
Associate Professor, Department of Microbiology, M.G.M. Medical College, Indore, MP, India
DOI: https://dx.doi.org/10.18535/jmscr/v6i10.77
