Title: Comparative study of three diagnostic techniques for diagnosis of malaria at a Tertiary care hospital in Kanpur

Authors: Beenu Prajapati, R. Sujatha, Nidhi Pal, Praveen Kumar Gautam

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i10.74

Abstract

Malaria continues to be a global public health problem. Microscopic examination of peripheral blood smear is the standard method for malaria diagnosis, which is available easily and has low cost but its reliability is questionable at low level of parasitaemia. The present study was undertaken to compare three different methods for diagnosis of malaria, i.e. peripheral blood smear and Antigen card test. In this study Peripheral blood smear (PBS), centrifuged buffy coat smear (CBS) and Antigen card test (RDT) were compared with each other taking peripheral blood smear as gold standard.  

Materials and Methods: The study was carried out over a 12 month period from January 2016 to December 2016. Blood samples (3-4 ml per patients) collected in EDTA vials from patients with clinical presentation of malaria were subjected to perform peripheral blood smear, buffy coat smear and rapid card test for the diagnosis of malaria then results were compared taking PBS as the gold standard.

Result: Out of 144 samples malaria was diagnosed in 53(36.80%), 69(47.91%) and 70(48.61%) by PBS, CBS and RDT respectively. The sensitivity of CBS and RDT was 76.81% and 75.71% respectively and specificity was 100%.

Conclusion: The development of easy, rapid and accurate test for the reliable detection of malaria infection is necessary. The centrifuged buffy coat smear technique fulfills most of these criteria and may be used at limited diagnostic source setup for low cost and reliable diagnosis of malaria.

Keywords: Malaria, Peripheral blood smear, Rapid card test.

References

  1. Yadav, M. Sharma and Aparnaet al. Comparative evaluation of pan-malaria antigen card test and blood smear for diagnosing malaria. Int. J. Life Sc. Btand Pharm Res.2012; 1(3): 56-58.
  2. Alonso. Malaria: developing a candidate vaccine (RTS,s/ASO2A) for an old source ofhumankind. International Journal of microbiology:2006;9:83-93
  3. World health organization. World malaria report 2015. Available from www.who.int/malaria/worldreport2015.
  4. National vector born disease control program, report, India 2015- 2016. Available from :www.nvbdcp.gov.in
  5. Singh et al; Prevalence of Malaria in a Tertiary Care Hospital in Navi Mumbai: J Bacteriolparasitol2015;6:2
  6. Phillips RS. Current Statusof Malaria and Potential for Control. Clinical Micro-biology Reviews 2001; 14 (1): 208-226.
  7. Suh KN, Kain KC and Keystone JS. Malaria. Canadian Medical Association Journal 2004; 170 (11), 1693-1703.
  8. Centre for Disease Control and Prevention. Malaria disease2015. Available at cdc.gov/malaria.
  9. Tierney LM, McPhee SJ and Papadakis MA. Malaria. In: Current Medical diagnosis and Treatment 49thedn. 2005: 1417-1426.
  10. Mankhambo L, Kanjala M, Rudman S, Lema VM and RogersonSJ. Evaluation of the Optimal Rapid Antigen Test and Species-Specific PCR to Detect Placental Plasmodium falciparum Infection at Delivery. Journal of Clinical Microbiology 2002; 40 (1): 155-158.
  11. S Mohanty et al; Usefullness of a centrifuged buffy coat smear examination for diagnosis of malaria. Indian journal of microbiology.2015; 33(1):63-67
  12. Cheesbrough M. District Laboratory practice. In: Tropical Countries, Part-1, Low price edn. Bath press and bounded in at the University press, Cambridge 1999; 239- 258.
  13. Akhtar et al; Feasible choice in diagnostic methods of malaria, J Vector Born Dis, September 2010; 47:151-155
  14. Malaria Roll Back. Malaria Diagnostics 2002. WWW.malaria. Am
  15. Malik GM, Seidi O, EL-TaherA, Mohammad AS. Clinical Aspects of Malaria in the Asir Region Saudi Arabia. Annual Saudi Medicine 1998; 18 (1): 15-17
  16. Estacio RH, Edwin ER. Dy, Cresswell S, Coronel RF. and Alora AT. The Quantitative Buffy Coat Technique (QBC) in Early Diagnosis of Malaria: The Santo Tomas University Hospital Experience. Philippines Journalof Microbiology and Infectious  Disease 1993; 22 (2): 56-59.
  17. Annobil SH, Okeahialam TC, Jamjoom GA, Bassuni WA. Malaria In Children-Experience From Asir Region, Saudi Arabia. Annual Saudi Medicine 1994; 14 (6): 467-470
  18. Annette AM, Philip Kruger et al. Malari incidence in Limpopo province, South Africa.2008; 7;162
  19. Shambhavi Singh, Vivek Agwanet al. Usefulness of Modified Centrifuged Blood Smear in Diagnosis of Malaria. Int. J. Curr. Microbiol. App.Sci2016; 5(3): 764-769
  20. Jafar Ebrahimet al. Comparative study of Thick Smear, Thin Smear, QBC abd Antigen Card Test in Diagnosis of Malaria. Int.J. Pure Appl. Sci. Technol 2013;17(1):54-59.

Corresponding Author

Praveen Kumar Gautam

Postal Address: Senior Resident Hostel, Govt. Medical College, Kannauj, India

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