Details
Category: Volume 06 Issue 10 October 2018
Hits: 1052

Title: Serum Profile of Selected Oxidants and Antioxidants in Patients of Rheumatoid Arthritis

Authors: Sangeeta Singh, Basant Joshi, Ziledar Ali

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i10.29

Abstract

The level of selected markers of oxidative stress (protein carbonyls, nitrite, malondialdehyde) and antioxidants (ceruloplasmin and ascorbic acid) were measured in the serum of 40 patients with rheumatoid arthritis. 20 healthy individuals were taken as controls. All the markers of oxidative stress were seen to be significantly elevated in patients of rheumatoid arthritis as compared to controls (p <0.001). Among the antioxidants, while serum ceruloplasmin was significantly raised in patients (p <0.001) as compared to controls, there was no significant difference in serum ascorbic acid levels between the two groups. A significant positive correlation was observed between ceruloplasmin and all the measured indicators of oxidative stress in patients of rheumatoid arthritis (r = 0.3 p<0.05 for protein carbonyls, r = 0.57 p<0.01 for nitrite, r = 0.64 p<0.01 for malondialdehyde). No such correlation was observed in the control group.

Keywords: Protein carbonyls, nitrite, malondialdehyde, ceruloplasmin, ascorbic acid.

References

  1. Albani, S. and Carson, A.D. (1997) Etiology and pathogenesis of rheumatoid arthritis. In: Arthritis and Allied Conditions, 13th edn, Ed. Koopman, W.J. Williams and Wilkins, Baltimore, vol-1, p979-991.
  2. Shacter, E. (2000) Quantification and significance of protein oxidation in biological samples. Drug Metab Rev., 32, 307- 326.
  3. Stefanovic-Racic, M., Stadler, J. and Evans, C.H. (1993) Nitric oxide and arthritis. Arthritis Rheum., 36, 1036-1044.
  4. Moshage, H., Kok, B., Huizenga, J.R. and Jansen, P.L.M. (1995) Nitrite and nitrate determinations in plasma: a critical evaluation. Clin Chem., 41, 892-896.
  5. Halliwell, B. and Gutteridge, J.M.C. (1990) The antioxidants of human extracellular fluids. Arch Biochem Biophys., 280, 1.
  6. Reznik, A.Z. and Packer, L. (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. In: Methods in Enzymology. Academic press, San Diego, Vol 233, p 357-363.
  7. Satoh, K. (1978) Serum lipid peroxide in cerbrovascular disorders determined by a new colorimetric method. Clinica Chemica Acta., 90, 37-43.
  8. Ravin, H.A. (1961) An improved colorimetric enzymatic assay of ceruloplasmin. J Lab and Clin Med., 58, 161-168.
  9. Gowenlock, A.H. (1988) Vitamins. In: Practical Clinical Biochemistry,6th edn. Ed. Gowenlock, A.H. Heinemann medical books, London, p924-930.
  10. Lunec, J., Blake, D.R., McCleary, S.J., Brailsford, S. and Bacon, P.A.(1985) Self perpetuating mechanisms of immunoglobulin G aggregation in rheumatoid    J  Clin Invest., 76, 2084-2090.
  11. Jasin, H.E. (1987) Cross-linking of immune complexes by human mononuclear phagocytes. , 11,  117-129.
  12. Farrell, A.J., Blake, D.R., Richard, M., Palmer, M.J. and Salvador, M. (1992) Increased concentrations of nitrite in synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic disease. Ann Rheum Dis.,51:1219-1222
  13. Bhogade, R.B., Suryakar, A.N., Katkam, R.V., Sardeshmukh, A.S. and Rathi, D.B. (2002) Oxidative stress in rheumatoid arthritis. Indian Medical Gazette., 2, 38-42.
  14. Murrell, G.A.C., Jang, D. and Williams, R.J. (1995) Nitric oxide activates metalloproteinase enzymes in articular cartilage. Biochem Biophys Res Commun 266,  15-21.
  15. Ozgunes, H., Gurer, H. and Tuncer, S. (1995) Correlation between plasma malondialdehyde and ceruloplasmin activity values in rheumatoid arthritis. Clin Biochem., 28, 193-194.
  16. Jasvinder K. Gambhir, Promod Lali, Anil K. Jain. (1997) Correlation between blood antioxidant levels and lipid peroxidation in rheumatoid arthritis. Clin Biochem., 30,351-355.
  17. Conforti, A., Franco, L., Menegale, G., Milanino,K.,Piemonte,G.and Velo,GP. (1983) Serum copper and ceruloplasmin levels in rheumatoid arthritis and degenerative joint disease and their pharmacological implications. Pharmacol Res Commune ., 15,859-67
  18. Biemond, P., Swaak, A.J.G. and Koster, J.F. (1984) Protective factors against oxygen free radicals and hydrogen peroxide in rheumatoid arthritis synovial fluid. Arthritis Rheum.,27,760-5
  19. Schorah, C.J. (1996) Total vitamin C, as ascorbic acid and DHA concentrations in plasma of critically ill patients. Am J Clin Nutr., 63, 760.
  20. Sahud, M.A. and Cohen, R.J.(1971) Effects of aspirin ingestion on ascorbic acid levels in rheumatoid arthritis.,1, 937-8

Corresponding Author

Basant Joshi

Department of Biochemistry, Govt. Medical College, Haldwani, India

Email: This email address is being protected from spambots. You need JavaScript enabled to view it.