Title: Antioxidant Profile of HIV Positive Subjects on HAART Attending ART Clinic in Asaba, Delta State

Authors: Nwachukwu O. Ekpete*, T. G. Davies, Holy Brown, E. O. Ibegbulem

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i10.27

Abstract

Highly Active Antiretroviral Therapy (HAART) has been found to be very effective in reduction of viral load and progression of HIV disease. It is however believed that this is at the expense of elevated reactive oxygen species (ROS) levels. There are however, not enough data to support this in Nigeria. The aim of this study was to determine the possible effects of Highly Active Antiretroviral Therapy (HAART) on the antioxidant profile of HIV positive patients who attended antiretroviral clinic in Asaba, Delta State, Nigeria. This study examined the modification in the antioxidant profile of HIV positive subjects on HAART treatment in Asaba as compared to the HIV positive subject HAART-naïve. One hundred (100) HIV positive patients on HAART and forty (40) HIV positive HAART-naïve patients who attended the Antiretroviral Therapy clinic in Asaba were studied. Antioxidant profile (Superoxide dismutase activity, Glutathione peroxidase activity) & electrolytes (Calcium, Phosphorus and Magnesium) were determined. The results showed a significant higher values (p<0.05) for the HAART treated subjects as against the HAART-naïve participants for SOD(HAART treated:18.65 ± 4.91U/ml vs. HAART-naïve:15.59 ± 3.59U/ml; p<0.0021), GPX(HAART treated:1.19 ± 0.69U/ml vs. HAART-naïve:0.77 ± 0.44U/ml; p<0.001), Calcium(HAART treated:2.59±0.53mm0l/L vs. HAART-naïve:1.97± 0.25mmom/L; p<0.0001), Magnesium(HAART treated:0.97 ± 0.23mmol/L vs. HAART-naïve:0.78 ± 0.17mmol/L; p<0.0001), while a significant decrease was observed for Phosphorous(HAART treated:0.92 ± 0.25mmol/L vs. HAART-naïve:1.15 ± 0.46mmol/L; p<0.039). These increase in SOD & GPX activities indicates that a substantial oxidative stress occurs in the patients that are being treated with HAARTs in the ART Clinic in Asaba while showing a positive effect on the Calcium, Phosphorus and Magnesium. Conclusively, this study contributes to evidences that HAART further increases the oxidative stress on the HIV positive patients on HAART and the data offers a guide to clinicians and other professionals on the care and management of HIV positive patients that are on HAART treatment in Asaba and by extension in Nigeria.

Keywords: Antioxidants, HIV, HAART, ART Clinic, Asaba.

References

  1. (2006). The World Report 2006: working together for health. Geneva: World Health Organization.
  2. (2013). World Health Statistics 2013. Geneva: World Health Organization.
  3. Haughey, N. J., & Mattson, M. P. (2002). Calciun Dysregulation and Neuronal Apoptosis by the HIV-1 Protein Tat and gp120. Journal of Acquired Immune Deficiency Syndrome, 55-61.
  4. Zunddorf, G. & Reiser, G. (2011). Calcium Dysregulation and Homeostasis of Neural Calcium in the Molecular Mechanisms of Neurodegenerative. Antioxidants & Signaling, 14(7), 1275-1288.
  5. Johane, P. A., Elaheh, A., Jenny, C., Irving, S. & Sharon, W. (1998). Oxidative stress and plasma antioxidant micronutrients in humans. The American Journal of Clinical Nutrion, 43-47.
  6. Delmas-Beauvieux M., Peuchant E., Couchouron A., Constans J., Sergeant C., Simonoff M., Pellegrin J., Leng B., Conri C. & Clerc M. (1996). The enzymatic antioxidant system in blood and glutathione status in human immunodeficiency virus (HIV)-infected patients: effects of supplementation with selenium or B-Carotene. American Society for Clinical Nutrition, 64, 101-107.
  7. Duggal S, Chugh, T. D. & Duggal A. K. (2012). HIV and Malnutrition: Effects on Immune System. Journal of Immunology Research, 1-8. doi:http://dx.doi.org/10.1155/2012/784740
  8. Bermingham, E. N., Hesketh, J. E., Sinclair, B. R., Koolaard, J. P. & Roy, N. C. (2014). Selenium-Enriched foods are more effective at increasing Glutathione Peroxidase (GPX) activity compared with Selenomethione: A Meta-Analysis. Nutrients, 4002-4031.
  9. (2016, April 22). www.asabaatl.org/about.html. Retrieved from Asaba Progressive Union: http://www.asabaatl.org
  10. Misra, H. P & Fridovich, I. (1972). The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple assay for Superoxide Dismutase. Journal of Biology and Chemistry, 247, 3170-3175.
  11. Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G. & Hoekstra, W. G. (1973). Selenium: biochemical role as a component of glutathione peroxidase. Science, 179, 588-590.
  12. Morin, L. G. (1974). Direct Colorimetric Determination of Serum Calcium with O-Cresophthalein Complexone. American journal of Clinical Pathology, 61, 114-117.
  13. Ngondi, J. L.; Oben, J.; Forkah, D. M.; Etame, L. H. & Mbanya, D. (2006). The effect of different combination therapies on oxidative stress markers in HIV infected patients in Cameroon. AIDS Research and Therapy, 3, 19-20.
  14. Sundaram, M., Saghayam, S., Priya, B. Venkatesh K. K. & Balakrishnan, P. (2008). Changes in antioxidant profile among HIV-infected individuals on generic highly active antiretroviral therapy in Southern India. International Journal of Infectious Diseases., 12, 61-66.
  15. Shahar, E., Pollack, S., Hedem, E., Hassoun, G. & Nager, R. (2008). Effect of HAART on salivary composition and oxidative profile in HIV-1 infected patients. Current HIV Research, 6, 447-451.
  16. Manda, K. R., Banerjee, A., Banks, W. A. & Ercal, N. (2010). Highly active antiretroviral therapy drug combination induced oxidative stress and mitochondrial dysfunction in immortalized human blood-brain barrier endothelial cells. Free Radicals Biology & Medicine, 801-810.
  17. Apostolova, N., Gomez-Sucerquia, L. J., Moran, A., Alvarez, A., Blas-Garcia, A. & Esplugues, J. V. (2010). Enhanced oxidative stress and increased mitochondrial mass during efavirenz-induced apoptosis in human hepatic cells. Brazlian Journal of Pharmacology, 160(8), 2069-2084.
  18. Muller, F; Svardal, A.M; Nordoy, I; Berge, R. K; Aukrust, P. & Froland, S. S. (2000). Virological and immunological effects of antioxidant treatment in patients with HIV infection. European Journal of Clinical Investigation, 30, 905-914.
  19. Atkuri, K. R.; Mantovani, J. J. & Herzenberg, L. A. (2007). N-Acetylcysteine-a safe antidote for cysteine/glutathione deficiency. Current Opinion in Pharmacology, 7(4), 355-359.
  20. Millea, P. J. (2009). N-acetylcysteine: multiple clinical applications. American Family Physician, 80(3), 265-269.
  21. Afridi, H. I., Kazi, T. G., Naeemullah, F. S., Arain, S. S. & Brahman, K. D. (2013). Evaluation of calcium, magnesium, potassium and sodium in biological samples of male human immunodeficiency virus patients with tuberculosis and diarrhea compared to healthy control subjects in pakistan. Clinical Laboratory, 539-550.
  22. Kuehn, E. W., Anders, H. J., Bogner, J. R., Obermaier, J., Goebel, F. D. & Schlondorff, D. (1999). Hypocalcaemia in HIV infection and AIDS. Journal of Internal Medicine, 245, 69–73.
  23. Akande, T.; Ogundeko, T.; Oyegbami, Y.; Obisesan, O.; Builders, M. & Adeniyi, O. (2016). Serum calcium and phosphorus levels of HIV/AIDS positive patients. World Wide Journal of Multidisciplinary Research and Development, 2(2), 49-52.
  24. Bruder, J. M., Guise, T. A. & Mundy, G. R. (2002). Mineral Metabolism In: Endocrinology & Metabolism. New York: McGraw-Hill.
  25. Drezner, M. K. (2002). Phosphorus homeostasis and related disorders in: Principle of Bone Biology. London: Academic Press.
  26. Yanagawa, N. & Lee, D. B. (1992). Renal handling of calcium and phosphorus in: Disorders of bone and mineral metabolism. New York: Raven Press Ltd.
  27. Favus, M. J. (2002). Intestinal absorption of calcium, magnesium and phosphorus in: Disorders of Bone Mineral Metabolism. Philaelphia: Lippincott.
  28. Stein, T. P.; Nutinsky, C.; Condoluci, D.; Schluter, M. D. & Leskiw, M. J. (1990). Protein and energy substrate metabolism in AIDS patients. Metabolism, 39, 876-881.
  29. Coodley, G. O.; Loveless, M. O. & Merrill, T. M. (1994). The HIV wasting syndrome: a review. Journal of Acquired Immune Deficiency Syndromes, 7, 681-694.
  30. Haug, C.; Muller, F.; Aukrust, P. & Froland, S. S. (1994). Subnormal serum concentration of 1,25-Vitamin D in human immunod-eficiency virus infection: correlation with degree of immune deficiency and survival. Journal Infectious Diseases, 169, 889-893.

Corresponding Author

Nwachukwu O. Ekpete

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