Abstract
Introduction: AgNOR, a synonym for silver stained nucleolar organizing regions (NORs) of DNA located on the short ends of acrocentric chromosomes are emphasized in actively proliferating cells. When compared with immunohistochemistry, AgNOR can give information related to the proliferation status of the tumours as inferred by ki67 other proliferation and apoptotic markers. In this study we compare the AgNOR staining patterns in prostatic carcinoma with the Gleason score and immunohistochemical markers Bcl-2.
Methods: The present study was done on 62 cases of Prostatic carcinoma. In this study we compare the AgNOR staining patterns in prostatic carcinoma with Gleason score and immunohistochemical markers Bcl-2.This study will be able to ascertain the efficacy of AgNOR staining as a substitute Bcl-2. Statistical analysis of mAgNOR count with Gleason score and Bcl-2 was done by SPSS software statistics By using Kendalls tau b test.
Results: mAgNOR shows statistically significant correlation with Gleason score and insignificant correlation with Bcl-2.
Conclusions: Silver staining of Nucleolar organizer regions is a useful method in identifying and grading of prostatic carcinoma. mAgNOR count show positive correlation with Gleason score so, AgNOR staining may substitute the proliferative marker IHC in lab where IHC facility is not available.
Keywords: AgNOR- Silver stained nucleolar organizing regions(NORs) mAgNOR – Mean AgNOR, Gleason score, carcinoma prostate.
References
- Ayres J.G, Crocker J.G,Skilbeck N.Q: Differentiation of malignant from normal and reactive mesothelial cells by the argyrophile technique for Nucleolar Organizer Region associated protein. Thorax 43; p.366-370;1998.
- Cheville J.C,Clamon G.H and Robinson R.A. Silver stained Nucleolar Organizer regions in the differentiation of prostatic hyperplasia, intra epithelial neoplasia, and adenocarcinoma Mod Pathol 3;P 586-590;1990.
- Deschenes J and Weidner N. Nucleolar Organizer Regions (NOR) in hyperplastic and neoplastic prostate disease. Am J Surg Pathol 14, p 1148-1155;1990.
- Ghazizadeh M, Sasaki Y, Oguro T and Aihara K. Silver staining of nucleolar organizer regions in prostatic lesions. Histopathology 19,p 369-372;1991.
- Gleason D.F. Histopathologic Gradig of Prostate Cancer: A perspective. Hum Pathol 23; p 273-279;1992.
- Mostofic F.K., Isabell A, Sesterhenn, Davis C.J. Prostatic Carcinoma, Problems in the interpretation of Prostatic biopsies. Hum pathol 23;p 223-240;1992.
- Miyake H, Muramaki M, Kurahashi T, et al:Expression of potential molecular markers in prostate cancer: correlation with clinicopathological outcomes in patients undergoing radicalprostatectomy. UrolOncol 2008;doi:10.1016/j.urolonc.2008.08.001.
- Orrell J.M, Evans A.T and Grant A. A critical evaluation of AgNOR counting in benign naevi ad malignant melanoma.J Pathol 163;p239-244; 1991.
- Hansen AB, Ostergard B. Nucleolar organiser regions in hyperplastic and neoplastic prostatic tissue. Virchows Arch A Pathol Anat Histopathol. 1990;417:9–13.
- McDonnell TJ, Tronocoso P, Brisbay SM et al. Expression with the emergence of androgen-independent prostate cancer. Cancer Res 1992; 52: 6940–4
- Apakama I, Robinson MC, Walker N et al. bcl-2 overexpression combined with p53 nuclear protein accumulation correlates with hormone refractory prostate cancer. Br J Cancer 1996:
- Bubendorf L, Sauter G, Moch H, et al: Prognostic significance of bcl-2 in clinically localized prostate cancer. Am J Pathol 148:1557, 1996.
Corresponding Author
Khethmal P
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DOI: https://dx.doi.org/10.18535/jmscr/v6i10.11
