Title: AgNOR Staining in Prostatic Carcinoma - Comparative Study with p53 and Gleason Score
Authors: Khethmal P., Togy Thomas, Joy Augustine
DOI: https://dx.doi.org/10.18535/jmscr/v6i9.160
Abstract
Introduction: AgNOR, a synonym for silver stained nucleolar organizing regions (NORs) of DNA located on the short ends of acrocentric chromosomes are emphasized in actively proliferating cells. When compared with immunohistochemistry, AgNOR can give information related to the proliferation status of the tumours as inferred by ki67 other proliferation and apoptotic markers. In this study we compare the AgNOR staining patterns in prostatic carcinoma with the Gleason score and immunohistochemical markers p53.
Methods: The present study was done on 62 cases of Prostatic carcinoma. In this study we compare the AgNOR staining patterns in prostatic carcinoma with Gleason score and immunohistochemical markers p53.This study will be able to ascertain the efficacy of AgNOR staining as a substitute p53. Statistical analysis of mAgNOR count with Gleason score and p53 was done by SPSS software statistics By using Kendalls tau b test.
Results: mAgNOR shows statistically significant correlation with Gleason score and p53.
Conclusions: Silver staining of Nucleolar organizer regions is a useful method in identifying and grading of prostatic carcinoma. mAgNOR count show positive correlation with Gleason score and p53 IHC so, AgNOR staining may substitute the p53 IHC in lab where IHC facility is not available.
Keywords: AgNOR- Silver stained nucleolar organizing regions (NORs) mAgNOR – Mean AgNOR, Gleason score, carcinoma prostate.
References
- Ayres J.G, Crocker J.G,Skilbeck N.Q: Differentiation of malignant from normal and reactive mesothelial cells by the argyrophile technique for Nucleolar Organizer Region associated protein. Thorax 43; p.366-370;1998.
- Cheville J.C,Clamon G.H and Robinson R.A. Silver stained Nucleolar Organizer regions in the differentiation of prostatic hyperplasia, intra epithelial neoplasia, and adenocarcinoma Mod Pathol 3;P 586-590;1990.
- Deschenes J and Weidner N. Nucleolar Organizer Regions (NOR) in hyperplastic and neoplastic prostate disease. Am J Surg Pathol 14, p 1148-1155;1990.
- Ghazizadeh M, Sasaki Y, Oguro T and Aihara K. Silver staining of nucleolar organizer regions in prostatic lesions. Histopathology 19,p 369-372;1991.
- Gleason D.F. Histopathologic Gradig of Prostate Cancer: A perspective. Hum Pathol 23; p 273-279;1992.
- Mostofic F.K., Isabell A, Sesterhenn, Davis C.J. Prostatic Carcinoma, Problems in the interpretation of Prostatic biopsies. Hum pathol 23;p 223-240;1992.
- Miyake H, Muramaki M, Kurahashi T, et al: Expression of potential molecular markers in prostate cancer: correlation with clinicopathological outcomes in patients undergoing radicalprostatectomy. Urol Oncol 2008; doi:10.1016/j.urolonc.2008.08.001.
- Orrell J.M, Evans A.T and Grant A. A critical evaluation of AgNOR counting in benign naevi ad malignant melanoma.J Pathol 163;p239-244; 1991.
- Hansen AB, Ostergard B. Nucleolar organiser regions in hyperplastic and neoplastic prostatic tissue. Virchows Arch A Pathol Anat Histopathol. 1990;417:9–13.
- Rubin S. J., Halahan D. E., Ashman C. R. et al., Two prostate carcinoma cell lines demonstrate abnormalities in tumor suppressor genes, J Surg Oncol, 1991, 46:31–36.
- Isaac W. B., Carter B. S., Ewing C. M., Wild type p53 suppresses growth of human prostate cancer cells containing mutant p53 alleles, Cancer Res, 1991, 51:4716–4720.
- Foster C. S., MC Loughlin J., Bashir I. et al., Markers of the metastatic phenotype in prostate cancer, Hum Pathol, 1992, 23:381–387
- Bookstein R., Macgrogan D., Hilsenbeck S. G. et al., P53 is mutated in a subset of advanced-stage prostate cancer, Cancer Res, 1993, 53:3369–3373.
- Kubota Y., Shuin T., Uemura h. et al., Tumor suppressor gene p53 mutations in human prostate cancer, Prostate, 1995, 27:18–24.