Details
Category: Volume 06 Issue 09 September 2018
Hits: 1225

Title: Anticoagulants Effect on the platelets Rich Plasma Growth Factors Levels

Authors: Dr Hoda Elhady Ahmed Abdelmoneim, Dr Ahmed Salah Abdalhaleem

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i9.124

Abstract

Background: Platelet-rich plasma (PRP) are platelets concentrates made of autogenous blood with a high number of platelets in a small volume of plasma. The main component that appears to be associated with the therapeutic effect in PRP is the presence of growth factors (GF). Variable techniques for PRP preparation produce variable amounts of growth factors.

Subjects and Methods: The study included a total number of 30 healthy males volunteer. We collected blood samples on lithium heparin, EDTA (ethylene diamine tetra acetic acid) and trisodium citrate as anticoagulants and counted platelets in whole blood and PRP which were prepared by single spin and double spin. We measured platelets derived growth factor-BB (PDGF-BB) and transforming growth factor beta-1(TGFB-1) in PRP and platelets poor plasma (PPP).

Results: We obtained high significant difference between PRP and PPP were prepared from samples on the different anticoagulants (p<0.01) for both TGFB-1 and PDGF-BB. No significant correlation between platelets counts and both TGFB-1 and PDGF-BB in PRP from the different anticoagulants samples (p>0.05). High significant difference was found between growth factors in the PRP were prepared by single spin and double spin (p<0.01) where TGFB-1 and PDGF-BB were higher in single spin PRP than that prepared by double spin.

Keywords: Anticoagulants, Platelets rich plasma, Growth factors.

References

  1. Foster TE, Puskas BL,  Mandelbaum BR,  Gerhardt MB et Rodeo SA: Platelet-rich plasma: from basic science to clinical applications. Am J Sports Med. 2009:37: 2259-72.
  2. Mazzucco L, Balbo V, Cattana E, Guaschino R et Borzini P: Not every PRP-gel is born equal. Evaluation of growth factor availability for tissues through four PRP-gel preparations: Fibrinet®, RegenPRP-Kit ®, Plateltex ® and one manual procedure. International Society of Blood Transfusion 2009:97:110-118.
  3. Marx RE: Platelet-Rich Plasma (PRP): What is PRP and what is not PRP? Implant Dentistry 2009; Vol.10 no,4, 225-228.
  4. Bhatia A, Ramya BS, Dayananda SB et Panchakshari BK: Comparison of different methods of centrifugation for preparation of platelet- rich plasma (PRP). Indian Journal of Pathology and Oncology 2016: October-December ;3(4);535-39.
  5. Andrae J, Gallini R et Betsholtz C: Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008: 22(10):1276-1312.
  6. Lee JW, Kwon OH, Kim TK, Cho YK, Choi KY, Chung HO, Cho BC, Yang JD et Shin JH: Platelet-rich plasma: quantitative assessment of growth factor levels and comparative analysis of activated and inactivated groups. Arch Plast Surg. 2013:40:530-35.
  7. Lubkowska A, Dolegowska B et Banfi G: Growth factor content in PRP and their applicability in medicine: Journal of biological regulators & Homeostatic. 2012: vol. 26, no.2 (S) 3-22.
  8. Queiroz da Silva L, Huber SC, Montalvão SA, Bassora FD, Vinicius  De Paula E et Annichino-Bizzacchi J: Platelet Activation is not crucial for platelet-rich plasma (PRP), when used as autologous therapeutic product, and could be lyophilized without any growth factor loss. Blood 2016:128:2639.
  9. Cho JM, Lee YH, Baek RM, et Lee SW: Effect of platelet-rich plasma on ultraviolet b-induced skin wrinkles in nude mice. J Plast Reconstr Aesthet Surg. 2010: 641:e31-e39.
  10. Landesberg R, Roy M et Glickman RS: Quantification of growth factor levels using a simplified method of platelet rich plasma gel preparation. J Oral Maxillofac Surg. 2000:58:297-300.
  11. Weibrich G, Kleis WK, Hafner G et Hitzler WE: Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg. 2002:30:97-102.
  12. Bausset O, Giraudo L, Veran J, Magalon J, Coudreuse J, Magalon G, Dubois C, Serratrice N, Dignat-George F et Sabatier F: Formulation and Storage of Platelet-Rich Plasma Homemade Product. BioResearch Open Access 2012: Vol. 1, no. 3, 115-123.
  13. Bocci V, Valacchi, G, Rossi, R, Giustarini D, Paccagnini E, Pucci AM et Di Simplicio P: Studies on the biological effects of ozone: 9. Effects of ozone on human platelets. Platelets 1999:10,110-116.
  14. Valacchi G et Bocci V: Studies on the biological effects of ozone: 10. Release of factors from ozonated human platelets. Mediators Inflamm. 1999:8,205-209.
  15. Anitua E, Sanchez M, Zalduendo MM, Dolors de la Fuente M, Prado R, Orive G et Andía I:  Fibroblastic response to treatment with different preparations rich in growth factors. Cell Prolif. 2009: 42:162-170.
  16. Weibrich G, Kleis WK, Hafner G, Hitzler WE et Wagner W: Comparison of platelet, leukocyte, and growth factor levels in point-of-care platelet-enriched plasma, prepared using a modified curasan kit, with preparations received from a local blood bank. Clin Oral Implants Res. 2003:14:357-362.
  17. Peysselon F et Ricard-Blum S: Heparin-protein interactions: From affinity and kinetics to biological roles. Application to an interaction network regulating angiogenesis. Matrix biology: journal of the International Society for Matrix Biology 2014:35, April,73-81.
  18. Ronaldo JF,  Silva NP, Haddad NF,  Lopes LS, Ferreira FD, Filho RB, Cappelletti PA, Mello W,  Cordeiro-Spinetti E et Balduino A: Platelet-rich Plasma obtained with different anticoagulants and their effect on platelet numbers and mesenchymal stromal cells behavior In Vitro: Stem Cells International 2016: Vol. 3, January,1-11.
  1. Fukaya M et Ito A: A New Economic Method for Preparing Platelet-rich Plasma: Plast Reconstr Surg Glob Open. 2014: June, Vol. 2, Issue 6, p e162.
  2. Qiao J, An N et Ouyang X: Quantification of growth factors in different platelets concentrates. Platelets. 2017: Vol.28, Issue 8, 774-778.

Corresponding Author

Hoda Elhady Ahmed Abdelmoneim

Egypt, Zkazik City, 4 Alhasn Bn Alhaithm Street, Alqaumia

Email: This email address is being protected from spambots. You need JavaScript enabled to view it., 00201140026110