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Category: Volume 06 Issue 09 September 2018
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Title: Evaluation of Cytokines Response in Plasmodium falciparum Infection among Adolescents in Port Harcourt, Nigeria

Authors: P. D. Iduh, T. C. Adias, Evelyn M. Eze

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i9.54

Abstract

This study evaluated the immunomodulatory pattern of cytokine in adolescent infected with plasmodium falciparum, using observational longitudinal design. The study was carried out in Nigeria National Petroleum Corporation Medical services Akpajo, Port Harcourt Rivers State, with the aim of evaluating the changes in cytokine level among adolescent with Plasmodium falciparum. A total of 91 adolescents participated in this study, comprising of 61 (67.0%) subjects with mild to moderate malaria infection and 30 (33.0%) non-infected as control. There were 49 male and 42 female adolescents in the study population. Cytokine level such as IL-6, IL- 8, IL-10 and TNF were assayed by using Elab science reagent ELISA method, while malaria parasite diagnosis was done using RDT, quantitative buffy coat for malaria parasite and thick blood film, infected subject were treated with antimalaria drug. There were statistically significant increase in the mean value of IL-6, TNF and IL-8 in test group compared to control group at baseline. Comparison of male and female showed statistically significant increase in TNF, and IL-8 for male compared to female. After treatment there were significant decrease in TNF, IL-6 and IL-8 P < 0.05. Gender effect showed statistically significant increase in TNF and IL-8 in male compared to female. Others showed no significance. This study reveals several changes in cytokine such as tumor necrosis, interleukin-6, interleukin-8 which were all increased in malaria infection and could exacerbate disease condition.

Keywords: Cytokines, Malaria, Adolescent, Port Harcourt.

References

  1. Murray, C. J., Rosenfeld, L.C., Lim, S. S., Andrews K.G., Foreman KJ, Haring, D., Fullman, N., Naghavi, M., Lozano, R. & Lopez, A. D (2012). Global malaria mortality between 1980 and 2010: a systematic analysis. Lancet, 379, 413–431.
  2. WHO (2016). World Malaria Report (2016.) Geneva: World Health Organisation. Available online at: http://www.who.int/malaria/publications/world-malaria-report-2016/report/en/Accessed on 27 July 2018.
  3. Gething, P. W., Casey, D. C., Weiss, D. J., Bisanzio, D., Bhatt, S., Cameron, E. & Kutz, M. J. (2016). Mapping Plasmodium falciparum mortality in Africa between 1990 and 2015. New England Journal of Medicine, 375(25), 2435-2445.
  4. Gebru, T., Lalremruata, A., Kremsner, P. G., Mordmüller, B. & Held, J. (2017). Life-span of in vitro differentiated Plasmodium falciparum gametocytes. Malaria Journal, 16(1), 330.
  5. Ajao, A. M., Idris, N. I., Sunday, O. J., Popoola, N. A., Oladipo, S. O. &Ladipo, E. F. (2017). Knowledge, attitude and practices about malaria among members of a university community in Kwara State, Nigeria. Animal Research International, 14(3), 2793-2803.
  6. Storm, J., & Craig, A. G. (2014). Pathogenesis of cerebral malaria—inflammation and cytoadherence. Frontiers in cellular and infection microbiology, 4, 100-109
  7. Rowe, J. A., Claessens, A., Corrigan, R. A. & Arman, M. (2009). Adhesion of Plasmodium falciparum-infected erythro-cytes to human cells: molecular mechanisms and therapeutic implications Expert Review of Molecular Medicine,11, 16 – 25.
  8. Smith, J. D., Rowe, J. A., Higgins, M. K. & Lavstsen, T. (2013). Malaria's deadly grip: cytoadhesion of Plasmodium falciparum-infected erythrocytes.  Microbiology,15, 1976–1983.
  9. Turner, L., Lavstsen, T., Berger, S. S., Wang, C. W., Petersen, J. E., Avril, M. (2013). Severe malaria is associated with parasite binding to endothelial protein C receptor. Nature, 498, 502–505.
  10. Lennartz, F., Adams, Y., Bengtsson, A., Olsen, R. W., Turner, L. &Ndam, N. T. (2017). Structure-guided identification of a family of dual receptor-binding PfEMP1 that is associated with cerebral malaria. Cell Host Microbe 21, 403–414.
  11. Stanisic, D. I., Fowkes, F. J., Koinari, M., Javati, S., Lin, E., Kiniboro, B. & King, C. L. (2015). Acquisition of antibodies against Plasmodium falciparum merozoites and malaria immunity in young children and the influence of age, force of infection, and magnitude of response. Infection and Immunity, 83(2), 646-660.
  12. Imoru, M. ,Shehu, U. A. Ihesiulor,,U.G. &Kwaru, A. H. (2013). Haematological changes in malaria infectected children in North-West Nigeria. Turkish Journal of Medical Science,  43 (5). 838-842
  13. Erhabor, O., Mohammad, H.J., Ahmed, H. M. & Ezimah, C. U.  (2014).  Effect of Plasmodium Parasitaemia on some Haematological Parameters in Children Living in Sokoto, North.
  14. Wu, X., Gowda, N. M., Kumar, S. & Gowda, D. C. (2010). Protein–DNA complex is the exclusive malaria parasite component that activates dendritic cells and triggers innate immune responses. The Journal of Immunology, 184(8), 4338-4348.
  15. Tatfeng, Y.M. & Agbonlahor, D. E. (2010). Age related cytokine profile in uncomplicated Plasmodium malaria infection. Lancet, 41(4), 23 – 29.
  16. Milner Jr, D. A., Whitten, R. O., Kamiza, S., Carr, R., Liomba, G., Dzamalala, C. & Taylor, T. E. (2014). The systemic pathology of cerebral malaria in African children. Frontiers in Cellular and Infection Microbiology, 4, 104-105.
  17. Sarangi, A., Mohapatra, P. C., Dalai, R. K. & Sarangi, A. K. (2014). Serum IL-4, IL-12 and TNF-alpha in malaria: A comparative study associating cytokine responses with severity of disease from the Coastal Districts of Odisha. Journal of Parasitic Diseases, 38(2), 143-147.
  18. Chaves, Y. O., Costa, A. G., Pereira, M. L. M., Lacerda, M. V. G., Coelho-dos-Reis, J. G., Martins-Filho, O. A. &Marinho, C. R. F. (2016). Immune response pattern in recurrent Plasmodium vivax malaria. Malaria Journal, 15(1), 445.
  19. Scarpioni, R., Ricardi, M. &Albertazzi, V. (2016). Secondary amyloidosis in autoinflammatory diseases and the role of inflammation in renal damage. World Journal of Nephrology, 5 (1), 66–75.
  20. Gazzinelli, R. T., Kalantari, P., Fitzgerald, K. A. &Golenbock, D. T. (2014). Innate sensing of malaria parasites. Nature Review of Immunology,14, 744–757.
  21. Leoratti, F.M., Durlacher, R.R., Lacerda, M.V., Alecrim, M.G., Ferreira, A.W., Sanchez, M.C. &Moraes, S.L. (2008). Pattern of humuroal immune response to Plasmodium falciparumblood stage in individuals presenting different clinical expression of malaria. MalariaJournal, 7, 186-190.
  22. Fowkes, F. J., Boeuf, P., & Beeson, J. G. (2016). Immunity to malaria in an era of declining malaria transmission. Parasitology 143(2), 139-153.
  23. Ifeanyichukwu, M. O., Okamgba, O. C., Amilo, G. I. &Nwokorie, E. A. (2017). Peripheral parasitaemia and its association with plasma cytokines levels in malaria-infected pregnant women in Aba, Abia state, Nigeria. African Journal of Infectious Diseases, 11(2), 54-61.
  24. Agwu, M. O. & Ernest, C. I. (2014). Issues, challenges and prospects of small and medium scale enterprises (SMEs) in Port-Harcourt city. European Journal of Sustainable Development, 3(1), 101-114.
  25. Cheesbrough, M. (2009) Parasitological Tests. Part 1: District Laboratory Practice in Tropical Countries, 2nd ed. Cambridge: University Press.
  26. Langhorne, J., Ndungu, F. M., Sponaas, A. M. & Marsh, K. (2008). Immunity to malaria: morequestions than answers. Nature Immunology, 9, 725–732.
  27. Rodrigo N. R, Josue da Costa L. J, Bruna de Paula F. F, Paulo R. Z. A, Arlete B, Fabio L. S, Fatima S, Dalma M. B. & Joseli de Oliveira F. (2014). Alterations in cytokines and haematological parameters during the acute and convalescent phases of Plasmodium falciparum and Plasmodium vivax Memorial Institute of Oswald Cruz, Rio de Janerio. 1–9.

Corresponding Author

P. D. Iduh

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