Title: Analysis of Premalignant and Malignant Changes in the Exfoliated Oral Mucosal Cells
Authors: Mani Madan, Shaleen Chandra, Vineet Raj, Ruby Dwivedi, Amit Thahriani
DOI: https://dx.doi.org/10.18535/jmscr/v6i6.87
Abstract
Tobacco and its end products are considered as the main etiological factors responsible for the pathogenesis of oral cancer. Various cytological alterations have been reported with tobacco usage like change in the nuclear size, cell size, nuclear to cytoplasmic ratio, nuclear shape etc. Of these parameters, the nuclear size, cytoplasmic size and their ratio have been shown to be significant. The objective of the present study is to assess the effect of tobacco chewing on buccal mucosa of 75 patients using cytomorphometry. Comparison was made between cytoplasmic area, nuclear area and the ratio between nuclear to cytoplasmic area of buccal squames of 25 normal subjects (N), 25 subjects using some form of tobacco for more than 5 years but not having any oral lesion (H) and 25 patients having habit of using tobacco for more than 5 years and having tobacco associated oral lesion (L). The statistical analysis of the result showed variation in the cytoplasmic area, nuclear area and ratio between nuclear and cytoplasmic area in the buccal smears taken from Normal, Habit and Lesional groups. Application of quantitative techniques to cytological smears could possibly improve the diagnostic value of oral exfoliative cytology.
Keywords: Cytomorphometry, exfoliated mucosal cells, premalignant lesions.
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