Title: The Effectiveness of Insect Growth Regulator (IGR) on the Growth and the Development of Aedes aegypti and Aedes albopictus in Tangerang City, Indonesia

Authors: Cecep Dani Sucipto, Didin Wahyudin, Heri Jati Santoso, Itha Latho, Asep Tata Gunawan

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i4.148

Abstract

Background: Dengue fever is a world health problem. It is endemic in more than 100 countries and threatens about 2.5 billion or 40% of people living in urban, suburban, and rural areas in both tropical and subtropical climates. Various mitigation measures have been undertaken, including vector control using mosquito nest eradication, selective abatization with larvicides, and mosquito fogging. Elimination using chemicals causes many problems. Another alternative to control the growth and development of Aedes sp. mosquito is needed. The use of insect growth regulator (IGR) is suggested to overcome this problem, since it does not affect the cleanliness of water, does not increase resistance, and considered safe.

The Objective: The objective of this research was the IE50, IE90, and LC50 concentration of pyriproxyfen to eliminate Aedes aegypti and Aedes albopictus larvae.

Method: The type of research used was laboratory experimental with a post-test design with only one group control design. This research was done through the actual test by creating dose variations of 1000 ppm of pyriproxyfen, which were as much as 0,14 mL; 0.196 mL; 0.274 mL; 0.384 mL; 0.538 mL; and 0.752 mL. The results of observation of larvae mortality were calculated by probit analysis to obtain the LC50 value of pyriproxyfen against Aedes aegypti and Aedes albopictus. The LC50 value obtained from probit calculation was replicated four times and used to find the value of LT50.

Results: Aedes aegypti and Aedes albopictus larvae after pyriproxyfen treatment and probit calculation showed inhibition of the growth Aedes aegypti larvae, indicated by IE50 value (95% Fiducial limits (FL)) of 0.17ppb (0.13-0.21) and IE90 (95% FL) of 0.68ppb (0.43-1.08). Inhibition of the growth of Aedes albopictus larvae was indicated by the value of IE50 (95% FL) of 0.11ppb (0.09-0.13) and IE90 (95% FL) of 0.39ppb (0.26-0.57). External morphological changes also occurred to Aedes aegypti and Aedes albopictus larvae after the exposure. Flaking of the cuticle to the thorax and swelling of both the head and the thorax were observed. Therefore, insect growth regulator has the potential to prevent the development of larvae into adult mosquitoes.

Conclusions: Pyriproxyfen can inhibit the growth of larvae. Pyriproxyfen LC50 concentration on Aedes aegypti larvae were 1.63ppm and on Aedes albopictus larvae was 1.56ppm. The use of pyriproxyfen causes external morphological changes to occur in larvae, pupae, and adult mosquitoes especially on the feet, whereas the proboscis and wings do not undergo morphological changes.

Keywords: Aedes aegypti, Aedes albopictus, Insect Growth Regulator (IGR), Larvae, Pyriproxyfen

References

  1. Ali, A., Nayar, J.K., De Xue, R., 1995, Comparative Toxicity of Selected Larvicides and Insect Growth Regulators to A Florida Laboratory Population of Aedes albopictus, Journal of the American Mosquito Control Association, 11(1),72-76.
  2. Anonim, 2007, Pyriproxyfen(200), http://www.fao org/ag/AGP/AGPP/pesticid /JMPR / /99 eva/25 pyriproxyfen.pdf download tanggal 8 Juli 2007.
  3. Belding, D.L, 1952, Textbook of Clinical Parasitology, Appleton Century Crofts, Inc, New York, 745.
  4. Chism, B.D, Apperson, C.S., Horizontal transfer of the Insect Growth Regulator pyriproxyfen to larval microcosms by Gravid Aedes albopictus and Ochloretatus triseriatus Mosquitoes in the Laboratory, Medical and Veterinary Entomology(2003)17:211-220.
  5. Cutwa , O’Meara, G. F., 2006, Photographic Guide to Common Mosquitoes of Florida,  Florida Medical Entomology Laboratory,  University of Florida, Florida.
  6. Depkes, 2007, Modul Pelatihan Bagi Pengelola Program Pengendalian Penyakit Demam Berdarah Dengue di Indonesia, Ditjen PP PL Depkes, Jakarta.
  7. Djakaria, S., 1998, Pendahuluan Entomologi dan Vektor Penyakit Virus, Riketsia, Spiroketa, dan Bakteri dalam Parasitologi Kedokteran editor Gandahusada S., Ilahude H. D., Pribadi W., Edisi ketiga, Fakultas Kedokteran Universitas Indonesia, Jakarta, 213.
  8. Dinkes Kota Tangerang, 2015, Arsip Data DBD di Kota Tangerang.
  9. Finney, D.J., 1971, Probit Analysis, Third edition, At the University Press, Cambridge.
  10. Gaubard, J., 2007, Juvenile Hormone Binding Proteins Importance in the JH action, Introductory paper no 168, Lund University http:/kem.ekol. lu.se/introyohann.
  11. Hoedojo, 1998, Morfologi, Daur Hidup dan perilaku nyamuk dalam Parasitologi Kedokteran editor Gandahusada S., Ilahude H. D., Pribadi W., Edisi ketiga, Fakultas Kedokteran Universitas Indonesia, Jakarta, 221-224.
  12. Hoedojo, Zulhasriel, 1998, Pengendalian Vektor dalam Parasitologi Kedokteran editor  Gandahusada S., Ilahude H. D., Pribadi W., Edisi ketiga, Fakultas Kedokteran Universitas Indonesia, Jakarta, 244-246.
  13. Kesumawati Hadi,U., Koesharto, 2006, Nyamuk dalam Hama Permukiman Indonesia: Pengenalan, Biologi dan Pengendalian editor Sigit, S.H, Kesumawati Hadi, U., Institut Pertanian Bogor, Bogor, 23-48
  14. Kusriastuti, R., 2007, Kebijaksanaan Penanggulangan Demam Berdarah Dengue dalam Simposium Nasional Demam Berdarah Dengue, aspek Biologi Molekuler, Patogenesis, Manajemen dan Pencegahan Kejadian Luar Biasa, Pusat Studi Bioteknologi, Universitas Gadjah Mada, Yogyakarta, 16 Mei 2007.
  15. Mardihusodo, S.J., 2004, Cara-cara Inovatif Pengamatan dan Pengendalian Nyamuk Vektor, DBD, Pusat Kedokteran Tropis, UGM, Yogyakarta.
  16. Munif, Amrul, 1997, Pengaruh Residu Pyriproxyfen 0,5% terhadap Larva Aedes aegypti Pada Berbagai Simulasi Wadah Air, Cermin Dunia Kedokteran Nomor 119, Jakarta.
  17. Ponlawat, A., Scott, J.G., Harrington, L.G., 2005, Insecticide Susceptibility of Aedes aegypti and Aedes albopictus across Thailand, J. Medical Entomology, 42(%),821-825.
  18. Rozendaal, 1997, Vector Control; Methods for Use by Individuals and Communities, WHO, Geneva, 38-39,123.
  19. Service, M. W., 1996, Medical Entomology for Students, First edition, Chapman&Hall, London.
  20. Siddal, J.B., 1976, Insect Growth Regulators and Insect Control: A. Critical Appraisal, Environmental Health Persp., Vol 14, 119-126.
  21. Sihuincha, M., Perea, E.Z., Rioz, W.O., Stancil, J.D., 2005, Potential Use of Pyriproxyfen for Control of Aedes aegypti (Diptera: Culicidae) in Iquitos, Peru, J. Med.Entomol, 42(4), 620-630.
  22. Sullivan, J., 2000, Environmental Fate of Pyriproxyfen, Environmental Monitoring and Pest Management Branch, Sacramento.
  23. Sutaryo, 2004, Dengue, Medika Fakultas Kedokteran UGM, Yogyakarta.
  24. Sutomo, S., 2003, Rencana Strategi Pencegahan &Pemberantasan Demam Dengue/Demam Berdarah Dengue 2004-2006, Ditjen P2M-PL Depkes R.I., Jakarta
  25. Tunaz, H., Uygun, N., 2004, Insect Growth Regulators for Insect Pest Control, Turk J. Agric For 28(2004), 377-387.
  26. Waris, L., 2005, Pengaruh pyriproxyfen terhadap vektor malaria Anopheles subpictus grassi asal Pulau Kalimantan dalam Seminar Regional Sulawesi, Palu, 29 September 2005.
  27. WHO, 1995, Report of the Informal Consultation on Aircraft Disinsection, Geneva.
  28. WHO, 2001, Report of The Fourth Whopes, Working Group Meeting, Review of IR3535; KBR3023;(RS)-Methoprene 20%EC, Pyriproxyfen 0,5% GR, and Lambda-Cyhalothrin 2,5%CS, WHO/CDC/WHOPES, Geneva, 50.
  29. WHO, 2005, Guidelines For Laboratory and Field Testing of Mosquito Larvicides, WHO Communicable Disease Control, Prevention, and Eradication, WHOPES, Geneva
  30. WHO, 2006, Pyriproxyfen, WHO specifications and Evaluations for Public Health Pesticides, Geneva.
  31. Widyana, 1997, Pengaruh Penerapan Metode Pengendalian Vektor Terhadap Populasi Pradewasa Aedes aegypti di Kecamatan Kasihan Bantul, Tesis, UGM, Yogyakarta.

Corresponding Author

Cecep Dani Sucipto

Minister of Health Polytechnic, Banten, Indonesia

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