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Category: Volume 06 Issue 02 February 2018
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Title: Study of Prevalence of Hemoglobinopathies by HPLC in Central Gujarat

Authors: Dr Neelam Shah, Dr Neema Rana, Supriya, Meena Daveshwar

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i2.46

Abstract

Objectives: To evaluate Prevalence and geographical distribution of various hemoglobinopathies in central Gujarat by using HPLC.

Method: Hemoglobinopathies were evaluated by prospective study on BIORAD variant of HPLC (High Performance Liquid Chromatography) system in tertiary care hospital, central Gujarat. Out of 5624 cases received in our laboratotry 994 show sickling solubility test positive. A total 1203 samples were analysed by HPLC for sickle cell and other suspected heamoglobinopathies at SSG Hospital over a period of 1 year from January 2016 to December 2016. The retention times (RT), proportion of hemoglobin (%) and the peak characteristics of all hemoglobin fractions were recorded. Many cases were advised for familial study.

Results: Among 5624 samples studied, abnormal hemoglobinopathies was seen in 1022(18.17%) cases, In which sickle cell trait (SCT) 681(12.10%), sickle cell disease (SCD) 272(4.83%), sickle thalassemia syndrome 38(0.63%), Beta thalassemia minor 20( 0.35%), Beta thalassemia major 5 (0.09%), HBD Punjab 2(0.035%), Sickle HBD Punjab 2(0.035%), Sickle HBE 1 (0.017%), B-Thalassemia Intermediate 1 (0.017%).

Conclusion: In our study sickle cell trait and disease were the most prevalent hemoglobinopathies. Ethinicity wise distribution shows Rath was/Vasavas have high incidence of haemoglobinopathies .but many other casts have low incidence of haemoglobinopathies. So for effective control programme we should include all the casts. HPLC is a simple, accurate tool in the early detection and management of various hemoglobinopathies.

Keywords: HPLC, Hemoglobinopathies, Thalassemia, sickle cell trait and sickle cell disease.

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Corresponding Author

Dr Neelam Shah

04 Shantapark, Behind Citizen Society Atmjyoti Ashram Road, Vadodara-390023

Contact Number- 9724318304, Email: This email address is being protected from spambots. You need JavaScript enabled to view it.