Title: Cytochemical Analysis of Immature Cells - Use As a Diagnostic & Prognostic Tool for Leukemia
Authors: Dr Pankaj Shinde, Dr C.V.Kulkarni, Dr Sachin Sharma
DOI: https://dx.doi.org/10.18535/jmscr/v6i1.182
Abstract
Aims & Objectives: Our study aims to evaluate the role of cytochemical staining as a simple and cheaper method to confirm the diagnosis and prognosis of leukemia and its classification.
Material & Methods: Fifty eight patients with suspected clinical and haematological leukemia visiting the central pathology laboratory of medical college were studied. The study used morphology, cytochemical staining of myeloperoxidase (MPO), Sudan black (SBB), periodic acid Schiff (PAS) stain and leukocyte alkaline phosphates staining of blood smear to confirm the diagnosis of leukemia.
Conclusion: In our study, cytochemistry helped in diagnosis of 62.5 % of AML, 84.2% of ALL, 14.3% of CML, none in CLL in addition to morphology in suspected cases of leukemia. Those leukemia have cytochemical positive lymphoblast have poor prognosis.
Keyword: leukemia, cytochemical staining, Thrombocytopenia.
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