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Category: Volume 05 Issue 12 December 2017
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Title: Antibiogram of Extended-spectrum beta-lactamase (ESBL) producing Pseudomonas aeruginosa isolates from pus samples

Authors: Soumya.S, Mahantesh.B.Nagmoti

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i12.25

Abstract

Pseudomonas aeruginosa is one of the most common opportunistic nosocomial pathogens, which causes a wide spectrum of infections and leads to increased mortality and morbidity. Due to indiscriminate use of antibiotics, significant changes in microbial genetic ecology is seen and this has led to the spread of multidrug resistance globally. The present study was undertaken to detect the extended spectrum β lactamases (ESBL) in Pseudomonas aeruginosa isolated from pus samples and to evaluate their susceptibility patterns. A total of 90 isolates of P.aeruginosa were analyzed to study their sensitivity patterns. The presence of the ESBL enzyme was detected by the phenotypictest-Double Disc synergy test. Out of 90 isolates of P.aeruginosa, 20(73.3%) were ESBL producers (of the 30 P.aeruginosa resistant to cetazidime). All the ESBL producing isolates were sensitive to Imipenemwhich is a carbapenem and is the drug of choice for treating infections caused by ESBL producing P.aeruginosa. Thus, we recommend a routine surveillance on antibiotic resistance in all hospitals so that control measures can be taken to prevent the spread of these strains in the hospitals at a very early stage itself.

Keywords: Pseudomonas aeruginosa, ESBL, Imipenem, DDST.

References

  1. Shahid M, Malik A, Sheeba. Multidrug resistant Pseudomonas aeruginosastrains harbouring R-plasmids and Amp C β-lactamases isolated from hospitalized burn patients in tertiary care hospital of North India. FEMS Lett 2003;228:181-6.
  2. Performance Standards for Antimicrobial Susceptibility Testing. Twentieth Informational Supplement, CLSI Document M100‑S20. Wayne, PA: Clinical and Laboratory Standards Institute; 2010.
  3. Paterson DL, Bonomo RA. Extended-spectrum β-lactamases: a clinical update.  Clin Microbiol Rev. 2005;18: 657–686.
  4. Naas T, Phiippon L, Poirel L, Ronco E, Nordmann P. An SHV-derived extended-spectrum β-lactamase in Pseudomonas aeruginosa.Antimicrob Agents Chemother 1999;43:1281-4.  
  5. Mugnier P, Dubrous P, CasinI, Arlet G, Collatz E. A TEM-derived extended-spectrum β-lactamase in Pseudomonas aeruginosa.Antimicrob Agents Chemother 1996;40:2488-93.
  6. Clinical laboratory standards institute. Performance standards for antimicrobial susceptibility testing. Sixteenth international supplement. CLSI document M100 - S 16, Wayne PA:2007.
  7. National Committee for Clinical Labo-ratory Standards. Performance standards for antimicrobial disk susceptibility tests, 7th Approved standard. NCCLS document M2-A7, Vol. 20 No. 1; Wayne PA: January 2000.
  8. Aggarwal R, Chaudhary U, Bala K. Detection of extended-spectrum beta-lactamase in Pseudomonas aeruginosa. Indian J Pathol Microbiol.2008 ;51(2):222-4.
  9. Hanberger H, Garcia-Rodriguez JA, Gobernado M, Goossens H, Nilsson LE, Struelens MJ. Antibiotic susceptibility among aerobic gram-negative bacilli in intensive care units in 5 European countries. French and Portuguese ICU Study Groups. 1999;281:67–71. 
  10. Babini GS, Livermore DM. Antimicrobial resistance amongst Klebsiella Collected from intensive care units in Southern and Western Europe in 1997-1998. J Antimicrob Chemother.  2000;45:183–9.
  11. Moland ES, Black JA, Ourada J, Reisbig MD, Hanson ND, Thomson KS. Occurrence of newer beta-lactamases in Klebsiella pneumoniae isolates from 24 US hospitals. Antimicrob Agents Chemother. 2002;46:3837–42.
  12. Günseren F, Mamikoğlu L, Oztürk S, Yücesoy M, Biberoğlu K, Yuluğ N, et al. A surveillance study of antimicrobial resistance of gram-negative bacteria isolated from intensive care units in eight hospitals in Turkey. J Antimicrob Chemother. 1999;43:373–8. 
  13. El-Karsh T, Tawfik AF, Al-Shammary F, Al-Salah S, Kambal AM, Shibl A. Antimicrobial resistance and prevalence of extended spectrum β-lactamase among clinical isolates of gram-negative bacteria in Riyadh. J Chemother. 1995;7:509–14. 
  14. Borer A, Gilad J, Menashe G, Peled N, Riesenberg K, Schlaeffer F. Extended-spectrum beta-lactamase-producing Enterobacteriaceae strains in community-acquired bacteremia in Southern Israel. Med SciMonit. 2002;8:CR44–7. 
  15. Yu Y, Zhou W, Chen Y, Ding Y, Ma Y. Epidemiological and antibiotic resistant study on extended-spectrum betalactamase-producing Escherichia coli and Klebsiella pneumoniaein Zhejiang Province. Chin Med J (Engl) 2002;115:1479–82. 
  16. AitMhand R, Soukri A, Moustaoui N, Amarouch H, ElMdaghri N, Sirot D, et al. Plasmid-mediated TEM-3 extended-spectrum beta-lactamase production in Salmonella typhimuium in Casablanca. J Antimicrob Chemother. 2002;49:169–72. 
  17. Das A, Ray P, Garg R, Kaur B. Proceedings of the Silver Jubilee Conference.New Delhi: All India Institute of Medical Sciences; 2001. Extended spectrum beta-lactamase production in Gram negative bacterial isolates from cases of septicemia.
  18. Hansotia JB, Agarwal V, Pathak AA, Saoji AM. Extended Spectrum-lactamase mediated resistance to third generation cephalosporins in Klebsiella pneumoniae in Nagpur, central India. Indian J Med Res. 1997;105:158–61.
  19. Jarlier V, Nicolas MH, Fournier G, Philippon A. Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: Hospital prevalence and susceptibility patterns. Rev Infect Dis. 1988;10:867–78.
  20. Thomson KS, Sanders CC. Detection of Extended spectrum beta lactamases in the members of the family Enterobacteriaceae: Comparison of the double disc and three -dimensional test. Antimicrob Agents Chemother. 1992;36:1877–82. 
  21. Cormican MG, Marshall SA, Jones RN. Detection of ESBL producing strains by the Etest ESBL screen. J ClinMicrobiol. 1996;34:1880–4. 
  22. Abigail S, Mathai E, Jesudason MV, John TJ. Ceftazidime resistance among Klebsiella pneumoniae in South India. Indian J Med Res. 1995;102:53–5.
  23. Moland ES, Thomson KS. Extended spectrum beta lactamases of Enterobacteriaceae. J Antimicrob Chemother. 1994;33:666–8.
  24. Coudron PE, Moland ES, Sanders CC. Occurrence and detection of ESBL in members of the family Enterobacteriaceae at a veterans medical center. J Clin Microbial. 1997;35:2593–7.
  25. Datta P, Thakur A, Mishra B, Gupta V. Prevalence of clinical strains resistant to various beta lactamase in a tertiary care hospital in India. Jpn J Infect Dis. 2004;57:146–9.
  26. Jobayer M, Afroz Z, Nahar SS, Begum A, Begum SA, Shamsuzzaman SM. Antimicrobial susceptibility pattern of extended-spectrum beta- lactamases producing organisms isolated in a Tertiary Care Hospital, Bangladesh. Int J App Basic Med Res 2017;7:189-92.
  27. Begum S, Salam MA, AlamKhF, Begum N, Hassan P, Haq JA. Detection of extended spectrum ß‑lactamase in Pseudomonas spp. isolated from two tertiary care hospitals in Bangladesh. BMC Res Notes 2013;6:7.
  28. Farzana R, Shamsuzzaman SM, Mamun KZ, Shears P. Antimicrobial susceptibility pattern of extended spectrum beta‑lactamase producing gram‑negative bacteria isolated from wound and urine in a tertiary care hospital, Dhaka City, Bangladesh. Southeast Asian J Trop Med Public Health 2013;44:96‑103.

Corresponding Author

Dr Soumya.S

Assistant Professor, Department of Microbiology,

Jawaharlal Nehru Medical College, Belgaum

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