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Category: Volume 05 Issue 10 October 2017
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Title: Assessment of Biofilm Production in Carbapenem Resistant Acinetobacter Species Isolated from Different Clinical Specimens

Authors: Amrita Pattanaik, Banashankari G.S

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i10.108

Abstract

Introduction: Microbial biofilms are important virulence factors contributing not only to the severity of the disease process but also to the antibiotic resistance. Acinetobacter species, particularly Acinetobacter baumannii is an important pathogen causing nosocomial outbreaks of infection in healthcare settings. Herein, we evaluated the biofilm forming ability of Carbapenem resistant Acinetobacter isolated from various clinical specimens.

Methods: A total of 109 phenotypically identified Acinetobacter isolates from different clinical specimens were studied, in which, Carbapenem resistance was determined as per the CLSI guidelines. Those isolates were then quantitatively assessed for their biofilm forming ability using the Microtitre Plate Assay.

Results: Of the 109 Acinetobacter isolates studied, 64.2% were Carbapenem resistant. Among them, 77.1% were biofilm formers. There was significant association seen between Carbapenem resistance and biofilm formation (p = 0.024). Odds ratio calculated was 2.6.

Conclusion: Our study showed that among the Carbapenem resistant strains of Acinetobacter, a significant number were biofilm producers. Further genetic analysis may provide a better understanding of the virulence, multidrug resistance and survival of the bacteria in the hospital environment.

Keywords: Acinetobacter, Carbapenem resistant, Modified Hodge Test, Combined Disc Test, Microtitre Plate Assay, Biofilm.

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Corresponding Author

Banashankari G.S

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