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Category: Volume 05 Issue 09 September 2017
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Title: Use of Immunohistochemistry in Prostatic Carcinoma

Authors: Dr Jyothi C R, Dr Jisha K T, Dr Joy Augustine, Dr Simi S

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i9.115

Abstract

Introduction: Use of immunohistochemistry  in prostatic carcinoma

Materials and Method: All specimens of Prostatic needle biopsy received during the period of 9 months from 2012 October to 2013 June in the Department of Pathology, GMC-Thrissur were included in the study. Primary diagnosis made on H&E sections. A total of 35 cases of histologically proven prostatic adeno carcinoma cases were selected. Histological diagnosis was based on architectural pattern, absence of basal layer, nuclear atypia and prominent nucleoli. After Gleason Grading and Scoring were done, Immunohistochemistry was performed on all cases using six antibodies which include HWCK /34βE12, CK5/6, p63 as basal cell specific antibodies which    gives negative staining in invasive prostate carcinoma,  AMACR, PSA, CKAE1/AE3 stains the malignant  cells hence positive staining indicated carcinoma ,PSA antibody to confirm prostatic origin of the neoplastic  cells. Relevant clinical details including PSA values were also collected.  Study was approved by the institutional ethical committee.                 

Result: All the three Basal cell markers were negative in 100% of adenocarcinoma cases hence are the best markers useful in the diagnosis of Carcinoma prostate. PSA and CKAE1/AE3 were positive in most of the cases hence maybe useful in the diagnosis of Carcinoma. AMACR was positive in one fifth cases only and hence is less useful than basal cell markers in the diagnosis of carcinoma prostate.

Conclusion: All the three Basal cell markers give 100% specificity in the diagnosis of Carcinoma prostate hence are the best markers useful in the diagnosis of Carcinoma prostate

Keywords: Carcinoma prostate, Immunohistochemistry, antibodies HWCK /34βE12, CK5/6, p63,PSA, CKAE1/AE3 and AMACR.

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Corresponding Author

Dr Jisha K T

Assistant Professor, Department of Pathology, Government Medical College, Thrissur Kerala, India

Mobile no: 9447883193. Email: This email address is being protected from spambots. You need JavaScript enabled to view it.