Title: Use of Immunohistochemistry in Prostatic Carcinoma
Authors: Dr Jyothi C R, Dr Jisha K T, Dr Joy Augustine, Dr Simi S
DOI: https://dx.doi.org/10.18535/jmscr/v5i9.115
Abstract
Introduction: Use of immunohistochemistry in prostatic carcinoma
Materials and Method: All specimens of Prostatic needle biopsy received during the period of 9 months from 2012 October to 2013 June in the Department of Pathology, GMC-Thrissur were included in the study. Primary diagnosis made on H&E sections. A total of 35 cases of histologically proven prostatic adeno carcinoma cases were selected. Histological diagnosis was based on architectural pattern, absence of basal layer, nuclear atypia and prominent nucleoli. After Gleason Grading and Scoring were done, Immunohistochemistry was performed on all cases using six antibodies which include HWCK /34βE12, CK5/6, p63 as basal cell specific antibodies which gives negative staining in invasive prostate carcinoma, AMACR, PSA, CKAE1/AE3 stains the malignant cells hence positive staining indicated carcinoma ,PSA antibody to confirm prostatic origin of the neoplastic cells. Relevant clinical details including PSA values were also collected. Study was approved by the institutional ethical committee.
Result: All the three Basal cell markers were negative in 100% of adenocarcinoma cases hence are the best markers useful in the diagnosis of Carcinoma prostate. PSA and CKAE1/AE3 were positive in most of the cases hence maybe useful in the diagnosis of Carcinoma. AMACR was positive in one fifth cases only and hence is less useful than basal cell markers in the diagnosis of carcinoma prostate.
Conclusion: All the three Basal cell markers give 100% specificity in the diagnosis of Carcinoma prostate hence are the best markers useful in the diagnosis of Carcinoma prostate
Keywords: Carcinoma prostate, Immunohistochemistry, antibodies HWCK /34βE12, CK5/6, p63,PSA, CKAE1/AE3 and AMACR.
References
- Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin 2012;62:10–29.
- Epstein JI, Yang XJ: Prostate biopsy interpretation.5th ed. Philadelphia, PA: Lippincott Williams & Wilkins. 2015.
- Allis Demas, Soriano Talban, Elena Lazar, Angela Board, Cordoba Lăzureanu,Diana Herman et al. Pseudobenign prostate carcinomas: causes of false-negative biopsy results. Rom J Morphol Embryol 2011, 52(3 Suppl):963– 974.
- Omar Hameed and Peter A. Humphrey. Immunohistochemistry in the diagnosis of minimal prostate cancer. Current Diagnostic Pathology 2006; 12: 279–291.
- Gladell P. Paner, Daniel J. Luthringer and Mahul B. Amin. Best Practice in Diagnostic Immunohistochemistry Prostate Carcinoma and Its Mimics in Needle Core Biopsies. Arch Pathol Lab Med. 2008; 132:1388–1396.
- Katia R. M. Leite, Miguel Srougi, Adriana Sanudo, Marcos F. Dall’oglio, Adriano Nesrallah,Alberto A. Antunes, Jose Cury, Luiz H. Camara-Lopes.The Use of Immunohistochemistry for Diagnosis of Prostate Cancer. International Braz J Urol. Vol. 36 (5): 583-590, September - October, 2010
- K Kumaresan, Nandita Kakkar, Alka Verma, Arup Kumar Mandal, Shrawan Kumar Singh and Kusum Joshi. Diagnostic utility of alpha-methylacyl CoA racemase (P504S) & HMWCK in morphologically difficult prostate cancer. Diagnostic Pathology 2010, 5:83.
- Elizabeth M. Genega, Brian Hutchinson, Victor E. Reuter, Paul B. Gaudin. Immunophenotype of High-Grade Prostatic Adenocarcinoma and Urothelial Carcinoma. Mod Pathol 2000; 13(11):1186–1191.
- Jeffrey S. Ross, Christine E. Sheehan, Hugh A. G. Fisher, Ronald P. Kaufman, Prabhjot Kaur, Karen Gray et al. Correlation of Primary Tumor Prostate-Specific Membrane Antigen Expression with Disease Recurrence in Prostate Cancer. Clin Cancer Res 2003;9:6357-6362.
- Jae Y Ro, Alberto G Ayala, Steven S Shen. Advances in Surgical Pathology. Prostate cancer. Lippincot Williams 2012
- Angela Queisser,Susanne A Hagedorn, Martin Braun,Wenzel Vogel, Stefan Duensing and Sven Perner. Comparison of different prostatic markers in lymph node and distant metastases of prostate cancer Modern Pathology (2015) 28, 138–145;
- World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of the Urinary System and Male Genital Organs Edited by John N. Eble Guido Sauter Jonathan I. Epstein Isabell A. Sesterhenn IARC Press Lyon,2004