Title: Rapid detection of Mycobacterium tuberculosis and Rifampicin Resistance in extra pulmonary samples using GeneXpert MTB/RIF assay
Authors: Dr Rakesh Kumar, Dr Kumar Vikram, Dr Swati Salila, Dr Shailesh Kumar, Dr Namrata Kumari, Dr Anima Xess, Dr S. K. Shahi
DOI: https://dx.doi.org/10.18535/jmscr/v5i8.183
Abstract
Background: GeneXpert MTB/RIF assay is efficient and reliable technique for the rapid diagnosis of extra pulmonary TB (EPTB), especially in smear negative cases. For rifampicin resistance detection, it provides accurate results and can allow rapid initiation of MDR-TB treatment.
Objectives: The aim of the study is to evaluate Gene Xpert MTB/RIF assay for rapid detection of Mycobacterium tuberculosis and rifampicin resistance in extra pulmonary samples.
Materials and Method: This study was done in the department of Microbiology, Indira Gandhi Institute of Medical Sciences, Patna during the period of February to July 2016. Extra pulmonary samples were collected from clinically suspected cases of extra pulmonary tuberculosis and were subjected for Z-N staining, fluorescent microscopy and Gene Xpert MTB/RIF assay.
Result: 123 extra pulmonary samples included 21 pus, 28 pleural fluids, 14 ascitic fluids, 7 CSF, 16 lymph nodes, 6 synovial fluid, 15 bronchoalveolar lavage (BAL) and 16 endometrial samples. Out of these, 20.3% patients were GeneXpert MTB/RIF Assay positive, 5.6% were positive on fluorescent microscopy and 4.0 % were ZN positive. Rifampicin resistance was found in pus sample, pleural fluid, and lymph node sample.
Conclusion: Gene Xpert MTB/RIF assay is rapid method for diagnosis of extra pulmonary tuberculosis as compared to conventional methods. Because of its simplicity, rapidity and sensitivity, this seems to be a very gorgeous tool for diagnosis of extra pulmonary tuberculosis from clinical samples.
Keywords: Extra pulmonary tuberculosis, Mycobacterium tuberculosis, Gene Xpert MTB/RIF assay, Rifampicin resistance.
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