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Category: Volume 05 Issue 08 August 2017
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Title: Evaluation of Method for Glycated Hemoglobin and Its Correlation with Microalbuminuria as Early Markers of Nephropathy in Type II Diabetes Mellitus

Authors: Khadija Umar Abdullahi, Uma Arora, Ravjit Sabharwal

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i8.37

Abstract

Diabetic nephropathy is the leading cause of end stage renal disease and mortality. The aim of the study was (i) to correlate between two methods of HbA1c estimation; the column chromatography and the immunoturbidimetric method and (ii) to evaluate microalbuminuria and glycated hemoglobin (HbA1c) as early risk markers of nephropathy in type II diabetes mellitus. The present study includes two parts; the first part consists of forty known diabetic patients in which the method comparison of HbA1c was studied. The second part of the study comprises of eighty-four known diabetic patients and thirty healthy control subjects in which the presence of HbA1c and microalbumin was estimated and correlated. Venous blood was obtained for HbA1c & plasma glucose for the first part of the study. For the second part, venous blood was obtained for HbA1c, and blood glucose, while their morning urine sample was obtained for detection of microalbuminuria. Statistical analysis was done using SPSS version 24. All p-values <0.05 were considered statistically significant. The two HbA1c methods were highly correlated (r=0.905). However, the mean of immunoturbidimetric method is slightly lower (5.23%) when compared with column chromatographic method (5.69%).Both the methods had good correlation with random blood sugar value.  Random Blood Sugar (RBS), HbA1c and microalbumin were the highest in diabetic patients (240.4±43.7 mg%), (9.96±1.21%),  and(108.5±36.2mg%) when compared with non-diabetic healthy control subjects (93.4±16.1mg%), (4.80±0.50% )and (8.1±2.4 mg%) respectively. Microalbuminuria and HbA1c had a significant correlation (r=0.626, p<0.01). Microalbuminuria also had a good correlation with duration of diabetes (0.764, p<0.01).

Keywords: Random Blood Sugar--RBS, Glycated Hemoglobin---HbA1c, Microalbumin, Nephropathy, Diabetes Mellitus(DM).

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Corresponding Author

Dr Uma Arora

Professor Biochemistry PIMS Jalandhar

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