Abstract

Introduction: Culex sp mosquitoes with several species have been proved as vectors of disease such as West Nile Virus disease, filariasis, Japanese encephalitis (inflammation of the brain) and St Louis encephalitis. The use of natural insecticides derived from plant extracts proven to be safer because it has short residue time as, after the application, the natural insecticide will break down into compounds that are harmless to humans and the environment.

Objective: This research aims at finding out the effect and the concentration of the juice of squeezed orange peel in killing larvae of Culex sp mosquito effectively.

Methods: This research is an experiment using a Completely Randomized Design (CRD) with 4 (four) replications on each larvae test with 25 mosquitos during each treatment. To determine the different effect on each treatment, the data were analyzed by using Kruskal-Wallis test followed by Probit regression (Dose-Response Analysis)  to determine the lethal concentration 50 (LC) values in calculating the ppm (parts per million) concentration of insecticide that can lead to the elimination of 50%  insect population. 
Result: The results of this study indicate that the sweet orange peel (Citrus sinensis) has the effect of against larvae of Culex sp instar III. The number of larvae Culex sp elimination from the application of the juice of sweet orange peel at a concentration of 1%, 2%, 3%, 4%, 5% are 2.25, 7.25, 14.5, 21.5, 25 respectively. There are significant differences between the test group were given juice of orange peel with different concentrations.

Conclusion: The concentration of the juice of sweet orange peel (Citrus sinensis OSB) effect on mortality of larvae of Culex sp instar III during  24 hours obtained the values of LC₅₀ = 2.370% and LC₉₀ = 4.332%. Thus, the higher the concentration of the juice of sweet orange peel, the more the number of Culex sp larvae  will die.

Keywords:  Juice of sweet orange peel, Larvae,  Culex sp mosquitoes, Elimination

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