Title: Urinary 8-Ohdg an Oxidative Stress Marker to DNA and Total Antioxidant Status levels in Oral Cancer Patients
Authors: Nakkeeran.M, Subash.P, Inmozhi Sivakamasundari.R, Kanakasabai.G
DOI: https://dx.doi.org/10.18535/jmscr/v5i6.162
Abstract
Recently there has been a significant shift in mortality rate from infectious diseases to non-communicable diseases. Oral cancer is one of the commonest malignancies in India. Approximately 60,000 newly diagnosed cases of oral cancer are reported to occur every year. Oxidative stress is known to be a major cause of development of many diseases attributed free radical injury, which is often neutralized by various endogenous and exogenous antioxidant systems. 8-hydroxy-2-deoxyguanosine (8-OHdG) is a sensitive marker oxidative stress in DNA that could be detected in urine, plasma and leukocytes. Very few studies from this region on oxidative DNA stress as assessed by urinary 8-OHdG, malondialdehyde (MDA), protein thiols and ferric reducing ability plasma (FRAP) in oral cancer subjects have been undertaken and hence the present study.
Objectives: To compare the serum levels of lipid peroxidation, total antioxidant status and protein thiols in patients with oral cancer and healthy controls. To evaluate whether the estimation of urinary 8-OHdG could serve as a marker to predict the extent of cellular oxidative stress in oral cancer patients.
Methods: The study included 60 volunteers, who were divided into 30 controls, and 30 early stage oral cancer patients who were further classified into newly diagnosed (n=15) and one year treatment follow-up oral cancer subjects (n=15). Random urine samples were collected and analyzed for the concentration of 8-OHdG. Serum lipid profile and lipid peroxidation, protein thiols and FRAP assay were performed by photometric assay methods. The urinary 8-OHdG and serum MDA levels were significantly higher in newly diagnosed oral cancer subjects than one year treatment follow up subjects. Atherogenic markers were significantly altered in oral cancer subjects. A significant correlation exists between urinary 8-OHdG and FRAP in both the groups of oral cancer patients.
Conclusions: Significant increase in the urinary 8-OHdG and other oxidative stress markers firmly suggest increased rate of oxidative DNA modifications in oral cancer subjects. The association between urinary 8-OHdG and FRAP in oral cancer patients suggest the preventive role of antioxidant on DNA modifications as induced by oxidative stress. In future, more research has to be focused to study gender variation, histological types, and different grades of tumour as well as survival. This would be taken up in our laboratory, with near future in a phased manner.
Key Words: Oral cancer, lipid profile, oxidative stress, antioxidant, 8-OHdG 8Hydroxy de oxygunosine, MDA, ferric reducing ability plasma (FRAP).
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