Title: Grouping of Clinical Isolates of Streptococci by Latex Agglutination in A Tertiary Care Centre

Authors: Dr Lancy J, Dr Anupama, Dr Thara Ann Jose, Dr Lakshmi Balaraman

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i5.224

Abstract

 Latex agglutination technique in an important procedure in clinical laboratories. It is a more rapid and technically less demanding than traditional extraction precipitation methods for the rapid and accurate identification of the Lancefied group of beta haemolytic streptococci. It can be used to detect Group D antigen is streptococci and enterococci as well as group A, B, C, F and G antigens.  It is a reliable method for grouping of the beta-haemolytic streptococci and enterococci whenever they are isolated from clinical specimens in culture on steep blood agar in clinical laboratories. A total number of 33 isolates obtained from various clinical specimens received in the 24 hours clinical microbiology laboratory at Govt. Medical College, Hospital, Thiruvananthapuram during a period of 6 months from July 2016 to December 2016 were subjected to Lancefield Grouping by Latex Agglutination test. Majority of the isolates were Group A streptococci 14 (42%) followed by Group B streptococci 6 (18%), Group D streptococci and group F streptococci (6%) and 7 isolates(21%)  were non-groupable.

Lancefield grouping of the beta – haemolytc streptococci by latex Agglutination immediately after their isolation in culture helps the clinician to start treatment at the earliest with appropriate antibiotic without any delay thus providing better patient care in invasive and noninvasive streptococcal infections and to prevent complication.

Keywords: Beta – haemolytic streptococci, streptococcus pyogeneslatex agglutination, Lancefied grouping

References

 1.      Harvey, C.L. Mellmurray, M.B. Eur.J. Clinical Microbiology 3:6:526 (1984)

2.      Holt et al (1004) Bergey’s manual of Determinative Bacteriology (9th Edition) Lippincott, Williams & Wilkins. ISBN 0-683-00603-7.

3.      Kohler W. (June 2007). The present state of species within the genera streptococcus and Enterococcus. International Journal of Medical Microbiology 297 (3):133-50.

4.      Cohen – Poradosu r, kasper DL. (2007). Group A streptococcus epidemiology and vaccine implications. Clin. Infect. Disease 45 (7):863-5.

5.      Streptococcal infections (Invasive Group A strep) New York City Department of Health and Mental Hygiene. Nov. 2012.

6.      Isenberg, Henry D. (1992) clinical micro-biology procedures handbook. Washing-ton, D.C: American Society of Microbiology.

7.      Damask LJ, Montoya O, Axeford JL. Rapid slide agglutination test for Lancefied grouping of streptococcal. Arch Pathol Lab Med 1979 Aug : 103 (9) 456-8.

8.      Kaufhold A and Ferrieri P. The Microbiolgic aspects, including and eterococcal infections. Infect Dis Clin North Am. 1993 June 7(2): 235-56.

9.      Facklam R. What happened to the streptococci overview of taxonomic and nomendature changes. ClinMircobiol Rev. 2002 Oct.: 15(4):613-30.

10.  Slifkin M, E, Engwall C, Puchet GR. Direct plate serological grouping of beta – haemolytic streptococci from primary isolation plates with the phadebact streptococcus test. J. ClinMicrobiol. 1978 April: 7(4):356-60.

Corresponding Author

Dr Lancy J

Department of Microbiology,

Govt. Medical College, Thiruvananthapuram, Kerala