Title: Enterococcus hirae, Unexpected Bacteria Detected in Rectal Swab of A Subject in Nduga District, Papua Province by 16s rDNA Sequencing System

Authors: Hotma Martogi Lorensi Hutapea, Ratna Tanjung, Vatim Dwi Cahyani, Yustinus Maladan, Hana Krismawati, Antonius Oktavian

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i5.160

Abstract

Enterococci are opportunistic pathogens known as a significant cause of nosocomial and community-acquired infections. Enterococcus faecalisand E. faeciumare the most common species identified in this infection. Another species, E. hirae is known to infect animals, and rarely isolated from human. The goal of this study was to characterize Enterococci derived a4 years old female rectal swab. The characterization was performed using MicroSeqIDTM500 sequencing system. DNA genomic was extracted from single colony and characterized using MicroSeqIDTM500 system. The sequence data was analyzed by homology analysis to MicroSeq libraryTM, genbank, and phylogenetic. The sequencing results showed that the isolate highly homolog to Enterococci sp. Phylogenetic analysis of 16S rDNA showed that the isolate was closely related to Enterococcus hirae.

Keywords: Enterococci, Enterococcus hirae, MicroseqIDTM500, 16S rDNA.

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Corresponding Author

Hotma Martogi Lorensi Hutapea

Institute of Research and Development for Biomedicine Papua

Jl. Kesehatan No.10 Dok II, Jayapura 99112, Provinsi Papua, Indonesia