Title: In Vitro Antioxidant Activity of Methanolic Leaf Extract of Tridax Procumbens and Tithonia Diversifolia
Authors: Okereke Stanly Chukwuma, Nwaogwugwu, Joel Caleb, Chukwudoruo Chieme Sunday
DOI: https://dx.doi.org/10.18535/jmscr/v5i5.91
Abstract
Objective: To analyze the antioxidant activities of the methanolic leaf extract of Tridax procumbens and Tithonia diversifolia.
Methods: This was done using various antioxidant models; 1,1-diphenyl-2-picryl hydroxyl (DPPH) quenching assay and superoxide anion(O2°°) scavenging assay. Phenolic contents, flavonoid contents and ascorbic acid contents were also estimated
Results: The results of this study revealed that Thithonia diversifolia possessed a significantly higher (P < 0.05) phenolic content (115.00
13.00mg/100g), flavonoid content (120.67 11.01mg/100g) quercetin equivalent (QE) and ascorbic acid content (55.00 4.58mg/100g) of vitamin C equivalent, when compared with the phenolic content (81.00 2.65mg/100g), flavonoid content (85.00 4.00mg/100g QE) and ascorbic acid content (67.33 
3.06mg/100g) of vitamin C equivalent, when compared with the phenolic content (81.00 + 2.65 mg/100g), flavonoid content (85.00 + 4.00 mg/100g QE) and ascorbic acid content(67.33 + 3.06 mg/100g of vitamin C equivalent) of Tridax procumbens respectively. The DPPH Quenching activity of the METP showed higher effective antioxidant activity at 80µg/ml and 100µg/ml to be 102.00 1.00 and 109.00 6.56 respectively which were significantly different when compared to the standard which had 90.00 2.65 and 94.00 2.00 at same concentrations. But the values of METD was observed to be 50.00 0.87 and 50.10 0.90 which decreased significantly compared to values of METP and that of BHT. The Superoxide Scavenging Activity of METP also showed significant increase at all levels of concentration of extracts when compared to the METD.
Conclusion: The result showed that the leaf methanolic leaf extract of Tridax procumbens shows more antioxidant activity than Tithonia diversifolia.
Keywords: Tridax procumbens L, Tithonia diversifolia, Antioxidant, total phenolic, Flavonoids, DPPH assay, Reducing power activity.
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