Title: Detection of the CD64 on Neutrophils and CD69 on Lymphocytes by Flowcytometry as a Marker for Early Diagnosis of Neonatal Sepsis

Authors: Dr Manal. M. Khadhim, Dr Nasma. N. Al-Hajjiah, Orass. M. Shaheed

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i4.01

Abstract

Background: Neonatal sepsis remains one of the main causes of mortality and morbidity despite the progress in hygiene, introduction of new and potent antimicrobial agents for treatment, and advanced measures for diagnosis. The diagnosis of sepsis is difficult because of non-specificity of clinical signs and symptoms and overlapping of symptoms with other noninfectious causes of systemic inflammation.

Aim: Identification the CD64 and CD69 markers on the surface of neutrophils and lymphocytes respectively by using flowcytometry assay to evaluate their role in the early diagnoss of bacterial neonatal sepsis.

Methods: This study was conducted on 75 neonates who were admitted to Maternity and Childhood Teaching Hospital at neonatal intensive care unit (NICU) at AL-Diwaniyah city, and 75 healthy neonates as a (control group). Flowcytometric analysis was done for detection of CD64 on neutrophils and CD69 on lymphocytes. One ml of EDTA treated blood was freshly processed and analyzed by flowcytometry within 24 hours.

Results: The median CD64 neutrophil and CD69 lymphocytes expression was significantly higher among both cases groups compared to healthy controls. In addition, the median for proven sepsis group was significantly higher than that of probable sepsis group.

Conclusion: Neutrophil CD64 is a highly sensitive and specific marker for neonatal sepsis. Prospective studies incorporating CD64 into a sepsis scoring system are warranted. However, the important issues of cost and availability are required to be evaluated in routine clinical setting.

Keywords: Neonatal sepsis, neutrophil CD64, CD69 lymphocytes, Flowcytometry.

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Corresponding Author

Dr Manal. M. Khadhim

Assistant Professor, Department of Medical Microbiology

College of Medicine, Al-Qadisiyah University, Diwaniyah, Iraq

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