Title: Using of Bleaching Powder for Cleaning Embalmed Cadaveric Bones
Authors: Dr Amitav Sarma, Dr Baneswar Baro, Dr Gautam Chandra Das, Dr Ambath D. Momin, Mr. Biraj Bhuyan
DOI: https://dx.doi.org/10.18535/jmscr/v5i1.44
Abstract
Introduction: A new technique for cleaning bone specimens from embalmed cadavers after the dissection to study bone anatomy.
Materials and Methods: Long bones from embalmed cadaver after completion of whole body dissection were taken and removed fleshy tissue manually as far as possible. A new method was used for cleaning the bone specimens. For that all collected long bones were divided into three groups (Gr.I, Gr.II and Gr.III.)
Gr.I- Bones were immersed into chlorine solution of 50%, 70% & 90% consecutively for 10 (ten) days in each solution
Gr. II- Bones were directly immersed into clean tap water for 30 days at 10 (ten) days interval.
Gr. III- Bones were buried under superficial surface of soil during rainy season continuously for 30 days.
Results: Use of chlorine solution was found to be one of the more effective methods for cleaning of long bones in terms of time, cost and hygiene. Bones of Gr. I were found to be cleaned properly without any morphological distortion. Bones of Gr. II were not cleaned at all while bones of Gr. III were partially cleaned. Discussion: Use of chlorine solution for cleaning bones collected from dissected embalmed cadaver for preparation of bone specimen has of immense importance, which is less time consuming, less laborious and more hygienic as well as very much cost effective technique.
Conclusion: By using this technique, medical institutes can collect bone specimen from the dissected cadaver within a short period of time for the teaching as well as research purpose.
Keywords: Bones, Cleaning, Embalming, Cadaver, Chlorine solution.
References
1. 1. Mafart B, JP Pelletier and M Fixot. Post-mortem ablation of the heart: a medieval funerary practice. International Journal of Osteoarchaeology. 2004; 14(10):67-73.
2. Vasudeva N, Mishra S. Inderbir Singh’s Text Book of Human Histology. 7th ed. New Delhi, London, Philadelphia, Panama: The Health Sciences publishers; 2014; 87-106.
3. Ajmani ML. Embalming- Principles and Legal Aspects. 1st ed. Jaypee Brothers Medical Publishers (P) Ltd., New Delhi;1998;111-118.
4. Voel D, Voel JG and Pratt CW. Fundamentals of Biochemistry: Life at the molecular level. Wiley and Sons, Hoboken, NJ. 2006;139-141.
5. Boskey AL, Cohen ML and Bullough PG. Hard tissue biochemistry: a comparison of fresh-frozen and formalin-fixed tissue samples. Calcif Tissue Int. 1982;34 (4):328-331.
6. Currey JD, Brear K, Zioupos P and Reilly GC. Effect of formaldehyde fixation on some mechanical properties of bovine bone. Biomaterials. 1995;16:1267-1271.
7. Nimni ME, Cheung D, Strates B, Kodamak M, Sheikh K. Chemically modified collagen- a natural biomaterial for tissue replacement. J. Biomed Mater Res. 1987; 21:741-771.
8. Cleaning methods - Seabird Osteology [Internet]. [cited 2015 April 29]. Available from: http://www.shearwater.nl/in-dex.php?file=kop131.php
9. By What Method the Bones and Cartilages of the Human Body May Be Prepared for Inspection [Internet]. [cited 2015 April 29].Available from: http://vesalius.northw-estern.edu/chapters/FA.1.39.html
10. Collecting, preserving and shipping specimens for the diagnosis of avian influenza A(H5N1) virus infection: World Health Organization 2006:Guide for field operations October 2006:39-41.
11. Calcium hypochlorite [Internet]. [cited 2015 April 19 ]. Available from: https://en.wikipedia.org/wiki/
12. [DOC]environment - National Institute of Health & Family Welfare [Internet]. [Cited 2015 April 30] Available from: www.nihfw.org/.../Public%20Health%20conclave/.../enviroment,occu%2...
13. Lawson T, Hopkins DW, Chudek JA, Janaway RC and Bell MG. Experimental earthwork at Wareham, Dorset after 33 years: 3: Interaction of soil organisms with buried materials. J. Archaeol. Sci.2000;27:273-285.
14. College of American Pathologist (CAP). CAP Handbook for Postmortem Examination of Unidentified Remains. CAP, Skokie, Illinois. 1986; 63-64.
15. Tibbet M and Carter DO. Soil Analysis in Forensic Taphonomy; Chemical and Biological Effects of Buried Human Remains. CRC Press, Florida.2008;1-66.
16. Kristen M, Hartnett , Laura C Fulginiti and Frank Di Modica. The Effects of Corrosive Substances on Human Bone, Teeth, Hair, Nails, and Soft Tissue. Journal of Forensic Sciences.2011; 56(4): 954–959.
17. Haaren EHV, Zwaard BCVD, Veen AJVD,Heyligers IC,Wuisman PIJM, Smit TH. Effect of long-term preservation on the mechanical properties of cortical bone in goats. Acta Orthopaedica 2008; 79 (5): 708–716.
18. Hildebrand M. Anatomical Preparations. Berkeley: California Press. 1968.
19. Van Gestel W. “Cleaning Skulls And Skeletons - By Maceration.” 2006. [cited 2015, May 5]. Available from: http://www.wkulsit.com/misc/macerationmanual.htm4.