Title: Anti Urolithiatic Activity of Rhus Mysorensis against Experimentally Induced Urolithiasis in Male Albino Rats
Authors: L. Sudheshna*, Sukesh Krishna Chaitanya Loka, A. Srinivasa Rao
DOI: http://dx.doi.org/10.18535/jmscr/v3i9.40
To evaluate the anti urolithiatic activity of Rhus mysorensis in experimentally induced urolithiasis in male wistar albino rats. The plant was shade dried at room temperature and coarsely powdered, extracted with 80% v/v alcohol. Male wistar albino rats were used for assessment of anti urolithiatic activity. Urolithiasis was induced in the animals using ethylene glycol and ammonium chloride. Standard drug cystone was used to treat standard group animals whereas ethanolic extract of Rhus mysorensis 200mg/kg b.w and 400mg/kg b.w were given to the test groups. Development of urolithiasis was confirmed by serum and urine biochemical estimations at the end of the experiment. Ethylene glycol and ammonium chloride developed calculi in the animals. Treatment with Rhus mysorensis reduced the formation of calculi. This was confirmed by biochemical estimations of urine and serum creatinine, calcium, oxalate, urea and uric acid. The mechanism underlying this effect is unknown but is apparently related to diuresis and lowering of urinary concentrations of stone forming agents. The protective effect against oxalate induced lipid peroxidation may be contributory to the recovery of renal damage. The present study revealed the anti urolithiatic activity of Rhus mysorensis against experimentally induced urolithiasis, reduced the formation of stones. Keywords: Ammonium chloride, Calculi, Ethylene glycol, Rhus mysorensis, Urolithiasis, 1. Kokate CK, Purohit AP, Gokhale SB. Text book of pharmacognosy. IVth ed. Pune: Nirali Prakashan; 1996. 2. Vogel HG, Similarities between various systems of traditional medicine: Considerations for the future of ethnopharmacology.J.Ethnopharmacol.,1991,35:179-90. 3. Ramarao AV, Gurjar MK.Drugs from plant resources: an overview .Pharma. Times 1990; 22 (5):19-21. 4. HandaSS. Future trends of plants as drugs. Pharma Times 1991; 23 (4): 13-23. 5. Nearing M, The green pharmacy. Herbal medicines in modern usage. IDRCR ep.,1985; 14(1): 10-1. 6. Silay M S, miroglu C. the risk of Urolithiasis recurrence may be reduced with anti nano bacterial therapy. Med hypotheses 2007; 68:1348-1350 7. Harkness, John E., Wagner, Joseph E., The Biology and Medicine of Rabbits and Rodents, Fourth Ed., Baltimore, Williams and Wilkins, 1995. 8. Madhava Shetty K. Flowering plants of chittoor district, 1stedition, and students offset printers, Tirupati, A.P., 2003; 150. 9. Deepak Reddy Gade, Sree Kumar Reddy G, Surya Narayana Reddy Akki, Vamsi Rajasekhar Reddy P, Hepatoprotective activity of Rhus Mysorensis against CCl4 induced hepatotoxicity in albino rats, “International Journal of Pharmaceutical Sciences Review and Research” Oct 2010, 46-48. 10. Dudekula, Noorulla Khadri, et al. "Evaluation of the hepatoprotective activity of Rhus mysorensis in albino rats." Indian Journal of Research in Pharmacy and Biotechnology 2.1 (2014): 1010. 11. Selvam R, Kalaiselvi P, Govindaraj A, Bala Murugan V and Sathish Kumar AS, Effect of Aerva lanata flowers extract and Vediuppu chunnam on the urinary risk factors of calcium oxalate urolithiasis during experimental hyperoxaluria, Pharmacol Res,2001, 43, 89-93. 12. Baggio B.etal.Calculi moxal at nephrolithiasis: an easy way to detect an Imbalance between promoting and inhibiting factors. Clinica. Chimica.Acta,1982;124(2):149-55 13. Thamilselvan S, Khan SR, Menon M. Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol Res 2003; 31:3–9. 14. Williams HE, Wandzilak TR. Oxalate synthesis, transport and the hyperoxaluric syndromes J. Urol., 1989; 141:742-7. 15. Tiselius HG. Medical evaluation of nephrolithiasis. Endocrinol Metab Clin North Am. 2002; 31:1031 50.Abstract
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