Title: Study of Insulin-Like Growth Factor-1 and Insulin-Like Growth Factor Binding Protein-1 Relation to Insulin Resistance in Normal Persons and Type 2 Diabetics
Authors: Khalifa A.M., Ismael A.A.,El Neily D.A., Abd El Ghafour R.A.
DOI: http://dx.doi.org/10.18535/jmscr/v3i11.03
Aim and Background: IGF binding proteins predominantly control the access of IGFs to tissues and cell-surface IGF receptors. Of these, IGFBP-1 is the most likely candidate for acute regulation of IGF actions because of its acute down regulation by insulin and upregulation by other glucoregulatory hormones and cytokines and in catabolic states. This study was undertaken to to assess the relation of the IGF-1 and IGFBP-1 to insulin resistance in a group of adult Egyptian diabetics and non diabetic population. Subjects and Methods:This study was conducted on 45 adult males with age between 20-50 years. They were divided into group I (n=25) type 2 diabetics and group II (n=20) non diabetics. Patient`s general information was noted, anthropometrical measurements were conducted and The eligible participants were asked to overnight fast for 8 hours. Blood was sampled for FBG, fasting Insulin, fasting IGF1, fasting IGFBP-1 And IR (HOMA-IR) was analyzed. Insulin and IGF-1 were analyzed by chemiluminescent immunoassay, FBG was analyzed by the glucoseoxidase (enzymatic oxidation) method and IGFBP-1 was analyzed by IGFBP-1 ElLISA Results: Diabetic group had IGF1(P=0.0001) and IGFBP-1(P=0.041)values statistically lower than non diabetic group. Diabetic group had a significant negative correlation between levels of FBG and those of IGF-1(p=0001).We noted that, diabetic group had a significant negative correlation between IGF-1 and IR(HOMA-IR) (p=.045) and a significant negative correlation between IGFBP1 and IR(HOMA-IR) (p=.046). Conclusion: IGF1 and IGFBP-1 seems to be at lower levels in Type 2diabetics than in normal subjects, IGF1 seems to be negatively associated with fasting glucose inType 2diabetics and Insulin resistance (HOMA-IR) seems to be negatively associated with IGF1 and IGFBP-1 in Type 2 diabetics. Keywords: IGFBP-1, IGF-1, IR(HOMA-IR), Type 2 diabetics 1. Daughaday WH, Rotwein P. 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